MAN1B1 Antibody (30-Y): sc-100543
- MAN1B1 Antibody (30-Y) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 100 µg/ml
- raised against recombinant MAN1B1 of human origin
- recommended for detection of MAN1B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MAN1B1 Antibody (30-Y). This work is in progress.
QUICK LINKS
MAN1B1 Antibody (30-Y) is a mouse monoclonal IgG2a antibody that detects MAN1B1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAN1B1, also known as mannosidase alpha, class 1B, member 1, is a crucial enzyme belonging to the glycosyl hydrolase 47 family, primarily localized in the endoplasmic reticulum (ER). This strategic location is vital as MAN1B1 plays a key role in the maturation of Asn-linked oligosaccharides on glycoproteins, which are essential for numerous biological processes such as cellular recognition, adhesion, and protein targeting. Furthermore, MAN1B1 is integral to the endoplasmic reticulum-associated protein degradation (ERAD) pathway, where MAN1B1 helps identify and direct misfolded or unassembled glycoproteins for degradation via the ubiquitin-proteasome system. MAN1B1′s activity is calcium-dependent and can be inhibited by specific class I alpha-mannosidase inhibitors like 1-deoxymannojirimycin and kifunensine, highlighting regulatory mechanisms and potential implications in glycoprotein quality control. With broad reactivity and versatile applications, anti-MAN1B1 antibody (30-Y) serves as an invaluable tool for researchers studying glycoprotein processing and related cellular functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAN1B1 Antibody (30-Y) References:
- N-Glycosylation of Serum IgG and Total Glycoproteins in MAN1B1 Deficiency. | Saldova, R., et al. 2015. J Proteome Res. 14: 4402-12. PMID: 26401844
- Clinical utility gene card for: MAN1B1 defective congenital disorder of glycosylation. | Jaeken, J., et al. 2016. Eur J Hum Genet. 24: PMID: 26577042
- MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer. | Wang, HF., et al. 2018. Gene. 679: 314-319. PMID: 30218751
- The cytoplasmic tail of human mannosidase Man1b1 contributes to catalysis-independent quality control of misfolded alpha1-antitrypsin. | Sun, AH., et al. 2020. Proc Natl Acad Sci U S A. 117: 24825-24836. PMID: 32958677
- MAN1B1-CDG: Three new individuals and associated biochemical profiles. | Sakhi, S., et al. 2021. Mol Genet Metab Rep. 28: 100775. PMID: 34141584
- MAN1B1-CDG: novel patients and novel variant. | Kasapkara, CS., et al. 2021. J Pediatr Endocrinol Metab. 34: 1207-1209. PMID: 34162022
- Translational balancing questioned: Unaltered glycosylation during disulfiram treatment in mannosyl-oligosaccharide alpha-1,2-mannnosidase-congenital disorders of glycosylation (MAN1B1-CDG). | Kemme, L., et al. 2021. JIMD Rep. 60: 42-55. PMID: 34258140
- Siblings with MAN1B1-CDG Showing Novel Biochemical Profiles. | Okamoto, N., et al. 2021. Cells. 10: PMID: 34831340
- Identification of MAN1B1 as a Novel Marker for Bladder Cancer and Its Relationship with Immune Cell Infiltration. | Wang, X., et al. 2022. J Oncol. 2022: 3387671. PMID: 36016584
- Hepatitis B virus X protein promotes MAN1B1 expression by enhancing stability of GRP78 via TRIM25 to facilitate hepatocarcinogenesis. | You, H., et al. 2023. Br J Cancer. 128: 992-1004. PMID: 36635499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAN1B1 Antibody (30-Y) | sc-100543 | 100 µg/ml | $333.00 |