MAN1A1 Double Nickase Plasmid (m): sc-421551-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAN1A1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAN1A1 Double Nickase Plasmid (m) and MAN1A1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Man1a. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAN1A1 Double Nickase Plasmid (m) | sc-421551-NIC | 20 µg | $410.00 |
Man1a encodes the Golgi-resident alpha-1,2-mannosidase MAN1A1, an enzyme that trims high-mannose N-glycans during glycoprotein maturation. This processing step contributes to N-glycan remodeling that influences protein folding, trafficking, and cell-surface glycoprotein composition, with downstream effects on secretion, receptor function, and cell–cell interactions. MAN1A1 activity interfaces with core glycosylation and quality-control processes along the secretory pathway and can modulate proteostasis and glycan-dependent signaling. Dysregulated N-glycosylation and altered mannosidase activity are commonly linked to phenotypes relevant to cancer biology, neurobiology, and immune regulation, supporting mechanistic studies of glycan processing in disease-relevant cellular states.
MAN1A1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Man1a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Man1a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Man1a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Man1a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.