MAD1 Antibody (D-6): sc-137026

MAD1 Antibody (D-6) is a mouse monoclonal IgG2b antibody that detects MAD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAD1 plays a critical role in cell cycle regulation by ensuring proper chromosome alignment at the mitotic spindle assembly checkpoint, preventing premature anaphase onset. This function is vital for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to cancer development. MAD1 is primarily localized in the nucleus, where MAD1 anchors MAD2L1 to the nuclear periphery, but during mitosis, MAD1 dynamically relocates to the centrosome, spindle midzone, and midbody, highlighting MAD1′s importance in the spatial regulation of mitotic events. Additionally, MAD1 can form homo- and heterodimers with MAD2, contributing to the formation of the tetrameric MAD1L1-MAD2L1 core complex, which is essential for the checkpoint′s functionality. MAD1 activity is further modulated by post-translational modifications, including hyperphosphorylation in response to spindle damage, underscoring MAD1′s role in the cellular response to mitotic stress. Defects in MAD1L1 are implicated in various cancers, making anti-MAD1 antibody (D-6) a valuable tool for studying cell cycle dynamics and cancer biology.

The preferred MAD1 antibody is MAD1 Antibody (9B10): sc-47746.