MacroH2A Double Nickase Plasmid (h): sc-407013-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MacroH2A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MacroH2A Double Nickase Plasmid (h) and MacroH2A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting H2AFY2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MacroH2A Antibody (C-9): sc-377452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MacroH2A Double Nickase Plasmid (h) | sc-407013-NIC | 20 µg | $410.00 | |||
MacroH2A Double Nickase Plasmid (h2) | sc-407013-NIC-2 | 20 µg | $410.00 |
H2AFY2 encodes macroH2A, a histone H2A variant that helps establish specialized chromatin states and modulates transcriptional output across the genome. MacroH2A deposition is linked to epigenetic memory, X chromosome–associated chromatin regulation, and stable gene repression, intersecting with pathways controlling cell cycle progression, lineage commitment, and stress-responsive transcription. By influencing nucleosome dynamics and chromatin accessibility, macroH2A contributes to regulation of DNA-templated processes including DNA repair and replication timing. Dysregulated macroH2A isoform balance and chromatin occupancy have been associated with altered differentiation programs and cancer-relevant transcriptional phenotypes, making H2AFY2 a target of interest in epigenetics and tumor biology research.
MacroH2A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the H2AFY2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within H2AFY2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt H2AFY2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of H2AFY2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.