mAChR M4 Double Nickase Plasmid (h): sc-402318-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mAChR M4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- mAChR M4 Double Nickase Plasmid (h) and mAChR M4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRM4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mAChR M4 Double Nickase Plasmid (h) | sc-402318-NIC | 20 µg | $410.00 | |||
mAChR M4 Double Nickase Plasmid (h2) | sc-402318-NIC-2 | 20 µg | $410.00 |
CHRM4 encodes the human muscarinic acetylcholine receptor M4 (mAChR M4), a Gi/o-coupled GPCR that inhibits adenylyl cyclase, reduces intracellular cAMP, and modulates ion channel activity to shape neuronal excitability and synaptic transmission. mAChR M4 signaling intersects with cholinergic and dopaminergic circuits and influences downstream pathways such as cAMP/PKA and MAPK/ERK, contributing to regulation of neurotransmitter release and plasticity. Altered CHRM4 expression or receptor function has been implicated in neuropsychiatric and neurodegenerative disease biology, including mechanisms relevant to schizophrenia, Parkinson’s disease circuitry, and substance use–related behaviors. These features make CHRM4 a useful target for dissecting GPCR signaling, network-level neuromodulation, and receptor-dependent transcriptional and electrophysiological phenotypes in human model systems.
mAChR M4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRM4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRM4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRM4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRM4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.