MAB21L Double Nickase Plasmid (h): sc-409644-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAB21L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAB21L Double Nickase Plasmid (h) and MAB21L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAB21L1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAB21L Double Nickase Plasmid (h) | sc-409644-NIC | 20 µg | $410.00 | |||
MAB21L Double Nickase Plasmid (h2) | sc-409644-NIC-2 | 20 µg | $410.00 |
MAB21L1 encodes MAB21L, a conserved nuclear protein implicated in transcriptional programs that coordinate embryonic development and cell fate decisions, with prominent relevance to ocular and craniofacial morphogenesis. Functional studies link MAB21 family members to regulation of differentiation, proliferation, and morphogen-driven patterning pathways, including processes downstream of BMP/TGF-β signaling. In human genetics, pathogenic variants in MAB21L1 are associated with congenital eye malformations and syndromic developmental phenotypes, highlighting its importance in developmental gene regulatory networks. These features make MAB21L1 a useful target for dissecting mechanisms of lineage specification and developmental disease biology in cellular models.
MAB21L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAB21L1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAB21L1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAB21L1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAB21L1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.