Date published: 2025-9-24

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m-IgG1 BP-HRP: sc-525408

4.8(4)
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Datasheets
  • mouse IgG1 binding protein (m-IgG1 BP) conjugated to Horseradish Peroxidase (HRP)
  • supplied at 100 µg in 0.5 ml volume
  • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
  • Suitable for binding to most, but not all, mouse IgG1 immunoglobulins; not suitable for use with mouse monoclonal IgG2a, IgG2b, IgG3, IgM, IgA or IgE antibodies. May slightly cross react with mouse IgG2b and goat IgG antibodies
  • Not cross-reactive with human, rat or rabbit IgG antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
  • Also see our new m-IgG1 BP-CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.

QUICK LINKS

Mouse IgG1 binding protein (m-IgG1 BP) conjugated to horseradish peroxidase (HRP) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB) and immunohistochemical (IHC) signal enhancement. Mouse IgG1 binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgG1 binding protein (m-IgG1 BP) is suitable for binding to most, but not all, mouse IgG1 immunoglobulins, comprising approximately 55% of SCBT's mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgG2a, IgG2b, IgG3, IgM, IgA and IgE antibodies. It may slightly cross react with mouse IgG2b and goat IgG antibodies. Not cross reactive with human, rat and rabbit IgG antibodies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgG1 BP-HRP

sc-525408
100 µg/0.5 ml
$92.00

Can the SCBT kappa-binding protein-HRP be use to detect sc-74399 (anti-Glut3 antibody) by western blot?

Asked by: josephine
Thank you for your question. kappa-binding protein-HRP can be used for western blot detection.We recommend a dilution range: 1:1000- 1:10000).
Answered by: BlakeJ
Date published: 2024-06-26

m-IgG1 BP-HRP secondary antiboty may be used for ELISA ?

Asked by: KIME
Thank you for your question. This secondary binding protein can also be used for solid state ELISA applications.
Answered by: Tech Support Europe
Date published: 2023-04-03

Are the mouse IgG binding proteins suitable for IHC on mouse tissue?

Asked by: Therése
Thank you for your question. If they match the properties of the primary antibody, secondary binding proteins can also be used for IHC on murine cells and tissues.
Answered by: Tech Support Europe
Date published: 2023-04-03
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Rated 5 out of 5 by from Great for chemiluminescent western blots!This detection reagent worked well for detecting PYK2 (E-3) antibody, sc-393181, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from ExcellentNow I am happy with that products. It’s giving good results
Date published: 2022-05-18
Rated 4 out of 5 by from pretty goodi bought this and was looking into CB1 CB2 along with beta Actin. I worked great with Beta Actin and CB2... I don't know it works with CB1 we are still working out the bugs but I don't think it really has anything to do with this secondary so really good, best first time try with SCBT! ;)
Date published: 2021-06-23
Rated 5 out of 5 by from worked fantasticvery useful to distinguish between different mouse originated Abs.
Date published: 2021-06-23
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m-IgG 1 BP-HRP is rated 4.8 out of 5 by 4.
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