Date published: 2026-2-21

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m-IgGκ BP-B: sc-516142

4.6(9)
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Datasheets
  • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to biotin (B)
  • supplied at 200 µg in 0.5 ml volume
  • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Immunohistochemistry (IHC)
  • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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Mouse IgGκ light chain binding protein is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for immunohistochemistry (IHC) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to biotin (B) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGκ BP-B

sc-516142
200 µg/0.5 ml
$93.00

Can we use normal goat serum as a blocking solution for immunohistochemistry when primary antibody is choactase (E-7): sc-55557, the secondary antibody is m-IgGκ BP-B: sc-516142 for rat brain free-floating sections?

Asked by: Sonali23
Thank you for your question. Yes, you can use normal goat serum for blocking for IHC experiments.
Answered by: Tech Service
Date published: 2019-03-01

Can this be used for ELISAs?

Asked by: Joyous
Thank you for your question. We have not tested this biotin-conjugated mouse IgG binding protein in an ELISA application so it is not a recommended application at this time. However, if you have a successful protocol using anti-mouse IgG conjugated to biotin then this product should also perform well in that protocol as it is comparable.
Answered by: Technical Support
Date published: 2018-11-20
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Rated 5 out of 5 by from Great for immunoperoxidase stainingGreat detection reagent for immunoperoxidase staining, pair with ImmunoCruz ABC kit sc-516216 for best results. For use with primary mouse monoclonal antibodies that bind to mouse IgGκ Binding Protein. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Great Secondary Antibody for Santa Cruz antibodiesGreat Secondary Antibody for Santa Cruz antibodies. However, I find it's difficult to strip off this protein from the membrane for reblotting. What do you recommend?
Date published: 2018-12-08
Rated 5 out of 5 by from IHC worksThe dilution 1:100 works good in IHC and can get good signal
Date published: 2018-01-01
Rated 5 out of 5 by from Detect the light chain of IgG in CO-IP productsThe antibody works good when diluted at 1:2000, and in Co-IP product, only light chain of IgG can be detected, which decreases the background signals.
Date published: 2017-11-02
Rated 5 out of 5 by from quite specificsilver staining indicates m-BP-B is also an antibody that won't catch much protein from HEK293T whole cell lysate
Date published: 2017-10-03
Rated 1 out of 5 by from Bad featuresWe used the antibody of your company a few months ago, but the result is very bad, we adjusted the experimental conditions and the proportion of the antibody, the results did not improve (pictures below), so we'd like to apply for a sample antibody , hope to be able to get your approval.
Date published: 2017-09-30
Rated 5 out of 5 by from worked wellI have used this antibody for the immunohistochemistry using the fixed brain sections, 1:500 dilution worked really well.
Date published: 2017-05-22
Rated 5 out of 5 by from worked wellI use this secondary antibody for the immunohistochemistry staining in the brain tissue. It worked well with 1:500 dilution
Date published: 2017-04-28
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m-IgGκ BP-B is rated 4.6 out of 5 by 9.
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