M-cadherin Antibody (5) recommended for detection of M-cadherin of mouse and rat origin by WB, IP and IF

M-cadherin Antibody (5): sc-136292

M-cadherin Antibody (5) is rated 5.0 out of 5 by 2.
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Datasheets

    • M-cadherin Antibody (5) is a mouse monoclonal IgG2a provided at 50 µg/0.5 ml
    • raised against amino acids 253-366 of M-cadherin of mouse origin
    • recommended for detection of M-cadherin of mouse and rat origin by WB, IP and IF
    • See M-cadherin (C-8): sc-398107 for M-cadherin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using M-cadherin (5): sc-136292 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Positive western blot analysis of M Positive western blot analysis of M-cadherin expression in C2C12 whole cell lysate and rat skeletal muscle and mouse cerebellum tissue extracts. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Positive result in western blot analysis of M Positive result in western blot analysis of M-cadherin expression in mouse neonate tissue extract. -SCBT QC
Date published: 2013-03-22
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