LYPLA2 Double Nickase Plasmid (h): sc-405133-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LYPLA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LYPLA2 Double Nickase Plasmid (h) and LYPLA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LYPLA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LYPLA2 Antibody (B-12): sc-390546
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LYPLA2 Double Nickase Plasmid (h) | sc-405133-NIC | 20 µg | $410.00 |
Human LYPLA2 encodes lysophospholipase II, a serine hydrolase that deacylates lysophospholipids and participates in membrane lipid remodeling and glycerophospholipid metabolism. Through regulation of fatty acyl turnover and lysophospholipid signaling pools, LYPLA2 influences organelle membrane dynamics, vesicular trafficking, and cellular stress responses. Altered phospholipid homeostasis is linked to inflammation, metabolic dysfunction, and proliferative phenotypes, making LYPLA2 a useful node for studying lipid-driven pathways and cell-state regulation. LYPLA2 activity is therefore relevant to investigations of bioactive lipid signaling networks and mechanisms connecting membrane composition to cellular physiology.
LYPLA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LYPLA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LYPLA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LYPLA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LYPLA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.