Ly6G5c Double Nickase Plasmid (h): sc-413393-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ly6G5c Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ly6G5c Double Nickase Plasmid (h) and Ly6G5c Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LY6G5C. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ly6G5c Double Nickase Plasmid (h) | sc-413393-NIC | 20 µg | $410.00 | |||
Ly6G5c Double Nickase Plasmid (h2) | sc-413393-NIC-2 | 20 µg | $410.00 |
LY6G5C encodes Ly6G5c, a member of the Ly6/uPAR superfamily of small, cysteine-rich proteins that are frequently associated with cell-surface or secreted signaling contexts. The gene resides within the MHC class III region on chromosome 6, linking it to genomic neighborhoods enriched for immune and inflammatory regulators. Although Ly6G5c is less well characterized than other Ly6 family proteins, reported patterns of expression and regional linkage suggest potential involvement in immune cell communication, epithelial–immune interactions, and modulation of inflammatory signaling networks. Genetic variation and dysregulated expression within the MHC locus have been associated with autoimmune and inflammatory disease susceptibility, making LY6G5C relevant for mechanistic studies of immune-related phenotypes.
Ly6G5c Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LY6G5C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LY6G5C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LY6G5C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LY6G5C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.