Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h): sc-402326-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h) and Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARHGDIB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12): sc-376473
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h) | sc-402326-NIC | 20 µg | $410.00 | |||
Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h2) | sc-402326-NIC-2 | 20 µg | $410.00 |
ARHGDIB encodes Ly-GDI/RhoGDI2, a Rho GDP-dissociation inhibitor that binds prenylated Rho-family GTPases such as RAC1, RHOA, and CDC42 to control their membrane association, activation cycling, and subcellular trafficking. By tuning Rho GTPase signaling, Ly-GDI influences actin cytoskeleton remodeling, cell adhesion and migration, vesicular trafficking, and NADPH oxidase–linked immune cell functions. ARHGDIB activity is therefore closely tied to pathways governing leukocyte chemotaxis, inflammatory signaling, and barrier interactions. Dysregulation of Rho GTPase control and ARHGDIB expression has been associated with altered immune cell behavior and disease-relevant phenotypes in hematologic and inflammatory research contexts.
Ly-GDI/RhoGDI2/ARHGDIB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARHGDIB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARHGDIB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARHGDIB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARHGDIB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.