Ly-6H Antibody (MN-7) recommended for detection of Ly-6H of human origin by WB, IP, IF, IHC(P) and ELISA

Ly-6H Antibody (MN-7): sc-100285

Ly-6H Antibody (MN-7) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-Ly-6H Antibody (MN-7) is a mouse monoclonal IgG1 (kappa light chain) Ly-6H antibody provided at 100 µg/ml
    • raised against recombinant Ly-6H of human origin
    • recommended for detection of Ly-6H of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ly-6H (MN-7): sc-100285.

Can sc-100285: Ly-6H (MN-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-100285: Ly-6H (MN-7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using K-562 whole cell lysate. -SCBT QC
Date published: 2015-07-11
Rated 2 out of 5 by from WB of transfected lysate was a bit messy WB of transfected lysate was a bit messy but detected a band a expected mw -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Produced superb immunoperoxidase membrane Produced superb immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2014-06-22
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