Ly-6G Antibody (RB6-8C5): sc-53515

Ly-6G Antibody (RB6-8C5) is a rat monoclonal IgG2b antibody that detects the Ly-6G protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Ly-6G (RB6-8C5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Ly-6G protein, also known as Lymphocyte antigen 6G, is a member of the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-anchored proteins, characterized by a distinctive cysteine-rich domain that contains O-linked carbohydrates. Ly-6G protein is predominantly expressed on neutrophils, where Ly-6G localizes to the cell membrane, playing a crucial role in the immune response. Notably, Ly-6G is part of the Gr-1 antigen complex, which is expressed on monocytes, neutrophils, and a subset of macrophages, as well as plasmacytoid dendritic cells and T cells. Ly-6G expression on these immune cells is significant as Ly-6G is involved in the regulation of immune responses, including the suppression of antitumor activity and T cell responses, making Ly-6G a valuable marker for studying neutrophil biology and their role in various pathological conditions.

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Ly-6G Antibody (RB6-8C5) References:

1. Absence of cross-reactivity between murine Ly-6C and Ly-6G.  |  Nagendra, S. and Schlueter, AJ. 2004. Cytometry A. 58: 195-200. PMID: 15057973
2. Neutrophils that infiltrate the central nervous system regulate T cell responses.  |  Zehntner, SP., et al. 2005. J Immunol. 174: 5124-31. PMID: 15814744
3. Altered CXCR2 signaling in beta-arrestin-2-deficient mouse models.  |  Su, Y., et al. 2005. J Immunol. 175: 5396-402. PMID: 16210646
4. Oncostatin M expression is functionally connected to neutrophils in the early inflammatory phase of skin repair: implications for normal and diabetes-impaired wounds.  |  Goren, I., et al. 2006. J Invest Dermatol. 126: 628-37. PMID: 16410783
5. The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection.  |  Koo, GC. and Gan, YH. 2006. BMC Immunol. 7: 19. PMID: 16919160
6. Capsule enhances pneumococcal colonization by limiting mucus-mediated clearance.  |  Nelson, AL., et al. 2007. Infect Immun. 75: 83-90. PMID: 17088346
7. Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites.  |  Tsou, CL., et al. 2007. J Clin Invest. 117: 902-9. PMID: 17364026
8. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.  |  Fleming, TJ., et al. 1993. J Immunol. 151: 2399-408. PMID: 8360469
9. Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins.  |  Fleming, TJ., et al. 1993. J Immunol. 150: 5379-90. PMID: 8515066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ly-6G Antibody (RB6-8C5) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-Ly-6G Antibody (RB6-8C5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ly-6G Antibody (RB6-8C5) Specifications

Alternate Product Name: Ly-6G

Product Clone Name: RB6-8C5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Granulocytes

Epitope Species: mouse

Product Reactivity/Detection: Ly-6G

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for Ly-6G Antibody (RB6-8C5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin