Anti-Ly-6G Antibody (RB6-8C5) is a rat monoclonal IgG2bLy-6G antibody provided at 200 µg/ml
raised against granulocytes of mouse origin
Ly-6G Antibody (RB6-8C5) is recommended for detection of Ly-6G of mouse origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Ly-6c
Anti-Ly-6G Antibody (RB6-8C5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ly-6G (RB6-8C5): sc-53515.
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Ly-6G Antibody (RB6-8C5) is a high quality monoclonal Ly-6G antibody (also designated Ly-6G antibody) suitable for the detection of the Ly-6G protein of mouse origin. Ly-6G Antibody (RB6-8C5) is available as both the non-conjugated anti-Ly-6G antibody form, as well as multiple conjugated forms of anti-Ly-6G antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Ly-6G (Lymphocyte antigen 6G) belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6G contains one UPAR/Ly6 domain and is expressed almost exclusively on neutrophils localizing to the cell membrane. Ly-6G together with Ly-6C make up the Gr-1 antigen. The Gr-1 antigen is ex- pressed on monocytes, neutrophils and a subset of macrophages, plasmacytoid dendritic cells and T cells. Cells expressing Gr-1 may play a role in the suppression of antitumor and T cell responses. Ly-6G is commonly used as a surface marker for neutrophils.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Will you please provide me with the protocol used to run the western blot for the Ly-6G Antibody (RB6-8C5): sc-53515 that was performed under non-reducing conditions. It would be most helpful to know the composition of the Transfer Buffer. Thank you.
Asked by: PeterMac
Thank you for your question. We followed our recommended protocol for Western blotting. Except, for the mouse PBL lysate we added our non-reducing electrophoresis buffer (sc-45085), and loaded on the regular SDS-PAGE gel without boiling. For our transfer buffer, we use sc-363874 Towbin powder, and add methanol at 10%. The bottle of Towbin powder makes 20L of 1x.
Answered by: Technical Support
Date published: 2019-08-17
How can Ly-6G (RB6-8C5): sc-53515 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?
Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53515 PE) or fluoroscein (sc-53515 FITC).
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