Ly-6G Antibody (RB6-8C5) recommended for detection of Ly-6G of mouse origin by WB, IP, IF, IHC(P) and FCM

Ly-6G Antibody (RB6-8C5): sc-53515

Ly-6G Antibody (RB6-8C5) is rated 4.4 out of 5 by 5.
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Datasheets

    • Anti-Ly-6G Antibody (RB6-8C5) is a rat monoclonal IgG2b Ly-6G antibody provided at 200 µg/ml
    • raised against granulocytes of mouse origin
    • Ly-6G Antibody (RB6-8C5) is recommended for detection of Ly-6G of mouse origin by WB, IP, IF, IHC(P) and FCM; may cross-react with Ly-6c
    • Anti-Ly-6G Antibody (RB6-8C5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ly-6G (RB6-8C5): sc-53515.

Will you please provide me with the protocol used to run the western blot for the Ly-6G Antibody (RB6-8C5): sc-53515 that was performed under non-reducing conditions. It would be most helpful to know the composition of the Transfer Buffer. Thank you.

Asked by: PeterMac
Thank you for your question. We followed our recommended protocol for Western blotting. Except, for the mouse PBL lysate we added our non-reducing electrophoresis buffer (sc-45085), and loaded on the regular SDS-PAGE gel without boiling. For our transfer buffer, we use sc-363874 Towbin powder, and add methanol at 10%. The bottle of Towbin powder makes 20L of 1x.
Answered by: Technical Support
Date published: 2019-08-17

How can Ly-6G (RB6-8C5): sc-53515 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53515 PE) or fluoroscein (sc-53515 FITC).
Answered by: Technical Support
Date published: 2017-12-04
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Rated 4 out of 5 by from Good product! We purchased this product a month ago and it worked nice for IHC staining.
Date published: 2018-07-10
Rated 5 out of 5 by from Great for immunohistochemical analysis I bought this antibody a month ago, and we use this antibody to mark the TANs isolated from the tumor site very well.
Date published: 2017-12-04
Rated 3 out of 5 by from Slight issue with background noise Using zinc-salt fixed mouse tumour section. Until now, I haven't convinced myself that the signal I've seen is actually Ly6G.
Date published: 2017-11-22
Rated 5 out of 5 by from A good antibody I am lucky to choose the good antibody, It is really a good antibody.
Date published: 2016-11-02
Rated 5 out of 5 by from Blow out band detected in mouse PBL tissue Blow out band detected in mouse PBL tissue extract under non-reducing conditions. - SCBT QC
Date published: 2014-07-14
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