Lucifer Yellow CH dilithium salt (CAS 67769-47-5)

Lucifer Yellow CH dilithium salt, bearing the CAS number 67769-47-5, is a highly fluorescent dye widely used in biological research to trace and visualize cells and cellular components. As a bi-functional dye, it is celebrated for its ability to be efficiently taken up by cells via pinocytosis, a form of endocytosis, where cells ingest extracellular fluid and its contents. This characteristic makes Lucifer Yellow particularly useful for studying cell morphology and the dynamics of vesicle trafficking within cells. Once inside the cells, the dye integrates into vesicles and can diffuse throughout the cytoplasm, providing a distinctive bright yellow-green fluorescence when excited by ultraviolet light. This makes it an invaluable tool in confocal microscopy and fluorescence microscopy studies, where it has been employed extensively to map out cell morphology, track cellular processes, and explain the behavior of networks of neurons. Researchers also benefit from its high photostability and water solubility, which contribute to its effectiveness in live-cell imaging over extended periods without significant photobleaching or toxicity. The compound′s ability to form a covalent bond with cellular components upon photoactivation adds another layer of utility, enabling detailed study of cellular architecture and connectivity in various types of tissues and cultured cells.