LRRC34 Double Nickase Plasmid (m): sc-428084-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRRC34 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRRC34 Double Nickase Plasmid (m) and LRRC34 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lrrc34. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRRC34 Double Nickase Plasmid (m) | sc-428084-NIC | 20 µg | $410.00 | |||
LRRC34 Double Nickase Plasmid (m2) | sc-428084-NIC-2 | 20 µg | $410.00 |
Mouse Lrrc34 encodes LRRC34, a leucine-rich repeat–containing protein implicated in protein–protein interaction networks that support cellular organization and signaling. Although its molecular partners remain incompletely defined, LRR-containing proteins commonly participate in scaffolding functions that influence cytoskeletal dynamics, membrane trafficking, and differentiation-associated transcriptional programs. Lrrc34 expression has been reported in germline and early developmental contexts, suggesting potential roles in stem/progenitor state regulation and lineage commitment. Dysregulation of leucine-rich repeat proteins is broadly relevant to developmental abnormalities and cancer-associated signaling rewiring, making Lrrc34 a useful target for mechanistic studies in these pathways.
LRRC34 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lrrc34 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lrrc34. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lrrc34 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lrrc34-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.