LRP6 Double Nickase Plasmid (m): sc-421466-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRP6 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRP6 Double Nickase Plasmid (m) and LRP6 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lrp6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRP6 Antibody (C-10): sc-25317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRP6 Double Nickase Plasmid (m) | sc-421466-NIC | 20 µg | $410.00 | |||
LRP6 Double Nickase Plasmid (m2) | sc-421466-NIC-2 | 20 µg | $410.00 |
Mouse Lrp6 encodes LDL receptor–related protein 6 (LRP6), a single-pass co-receptor that is essential for canonical Wnt/β-catenin signaling. Upon Wnt ligand binding, LRP6 participates in signalosome assembly with Frizzled receptors, promoting β-catenin stabilization and downstream transcriptional programs that regulate embryonic patterning, stem and progenitor cell behavior, and tissue homeostasis. LRP6 activity also intersects with pathways controlling cell polarity and receptor trafficking, shaping context-dependent signaling outputs. Dysregulated LRP6-dependent Wnt signaling has been linked to developmental abnormalities and altered tissue remodeling in multiple disease-relevant models, making Lrp6 a frequently interrogated node in pathway biology.
LRP6 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lrp6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lrp6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lrp6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lrp6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.