LRP1 Double Nickase Plasmid (m): sc-421464-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LRP1 Double Nickase Plasmid (m) and LRP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lrp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRP1 Antibody (8G1): sc-57353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRP1 Double Nickase Plasmid (m) | sc-421464-NIC | 20 µg | $410.00 | |||
LRP1 Double Nickase Plasmid (m2) | sc-421464-NIC-2 | 20 µg | $410.00 |
Low-density lipoprotein receptor-related protein 1 (LRP1), encoded by the mouse Lrp1 gene, is a multifunctional endocytic and signaling receptor that coordinates uptake of diverse ligands including lipoproteins, protease–inhibitor complexes, and extracellular matrix components. Through interactions with adaptor proteins and co-receptors, LRP1 influences clathrin-mediated endocytosis, cytoskeletal remodeling, and signal transduction pathways such as PI3K–AKT and MAPK, shaping cellular migration and survival. Lrp1 also modulates extracellular proteolysis by regulating uPA/uPAR and matrix metalloproteinase activity, linking receptor trafficking to tissue remodeling. Dysregulated LRP1 function has been associated with neurobiology, vascular and metabolic phenotypes, and tumor microenvironment processes, making it a widely studied node in homeostatic clearance and cell signaling networks.
LRP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lrp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lrp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lrp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lrp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.