Date published: 2025-12-18
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Western blot analysis of caspase-1 cleavage in untreated (A) and PMA (sc-3576) and LPS (sc-3535) treated (B) THP-1 whole c
Western blot analysis of caspase-1 cleavage in untreated (A) and PMA (sc-3576) and LPS (sc-3535) treated (B) THP-1 whole cell lysates. Antibody tested is caspase-1 (14F468): sc-56036 (A,B). Note cleavage of caspase-1 in lane B.
Western blot analysis of caspase-1 cleavage in untreated (A) and PMA (sc-3576) and LPS (sc-3535) treated (B) THP-1 whole c
Western blot analysis of caspase-1 cleavage in untreated (A) and PMA (sc-3576) and LPS (sc-3535) treated (B) THP-1 whole cell lysates. Antibody tested is caspase-1 (14F468): sc-56036 (A,B). Note cleavage of caspase-1 in lane B.
Western blot analysis of caspase-1 cleavage in untreated (A) and PMA (sc-3576) and LPS (sc-3535) treated (B) THP-1 whole cell lysates. Antibody tested is caspase-1 (14F468): sc-56036 (A,B). Note cleavage of caspase-1 in lane B.

LPS

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Alternate Names:
LPS is also known as Lipopolysaccharide.
Application:
LPS is a highly immunogenic major component of the cell wall of gram negative bacteria that strongly activates immune cells bearing the CD14/TLR4/MD2 receptor complex.
Purity:
purified by phenol extraction
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Description
Technical Information
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SDS & Certificate of Analysis

LPS (Lipopolysaccharide) is an endotoxin which exists as a critical glycolipid component of the outer cell wall of gram-negative bacteria. LPS is a known, potent initiator of the proinflammatory response and induces proinflammatory cytokines in several cell types. Many of the cell signals induced by gram-negative bacteria are attributed to LPS and has been observed to also activate TNF receptor signaling pathways. The major receptor that transduces the LPS signal has been recognized as a member of the Toll-like receptor family. At least 10 members of the family have been identified to date and TLR4 appears to be the main LPS receptor although coreceptors are most likely involved. Studies note that in HMEC (human microvascular endothelial cells) cell lines, LPS activates apoptosis pathways via both NF-kappaB and JNK through TRAF6. Also reported that NF-kappaB inhibition does not potentiate LPS-induced death in HMEC cells yet will sensitive these cell to TNF-induced death. Additional studies using HMEC cell lines report that following LPS stimulation, TRAF6 also transmits an important endothelial cell survival signal in a situation of complete NF-kappaB blockade which may indicate that TRAF6 signals an NF-kappaB–independent antiapoptotic pathway in endothelial cells exposed to LPS. LPS is also known as Lipopolysaccharides, Lipopolysaccharides from E. coli O111:B4, and Bacterial lipopolysaccharides.


LPS References

  1. Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes.  |  Zhang, FX., et al. 1999. J Biol Chem. 274: 7611-4. PMID: 10075645
  2. The lipopolysaccharide of moraxella catarrhalis structural relationships and antigenic properties.  |  Holme, T., et al. 1999. Eur J Biochem. 265: 524-9. PMID: 10504382
  3. Lipopolysaccharide signals an endothelial apoptosis pathway through TNF receptor-associated factor 6-mediated activation of c-Jun NH2-terminal kinase.  |  Hull, C., et al. 2002. J Immunol. 169: 2611-8. PMID: 12193732
  4. Lipopolysaccharide as a target for the development of novel therapeutics in gram-negative bacteria.  |  Yethon, JA. and Whitfield, C. 2001. Curr Drug Targets Infect Disord. 1: 91-106. PMID: 12455407
  5. Role of the MyD88 transduction signaling pathway in endothelial activation by antiphospholipid antibodies.  |  Raschi, E., et al. 2003. Blood. 101: 3495-500. PMID: 12531807
  6. Bacterial lipopolysaccharide directly induces angiogenesis through TRAF6-mediated activation of NF-kappaB and c-Jun N-terminal kinase.  |  Pollet, I., et al. 2003. Blood. 102: 1740-2. PMID: 12714497
  7. Lipopolysaccharide initiates a TRAF6-mediated endothelial survival signal.  |  Wong, F., et al. 2004. Blood. 103: 4520-6. PMID: 14996708
  8. Structural features and structural variability of the lipopolysaccharide of Yersinia pestis, the cause of plague.  |  Knirel, YA., et al. 2006. J Endotoxin Res. 12: 3-9. PMID: 16420739
  9. Lipopolysaccharide in bacterial chronic infection: insights from Helicobacter pylori lipopolysaccharide and lipid A.  |  Moran, AP. 2007. Int J Med Microbiol. 297: 307-19. PMID: 17467335
  10. Retinoic acid-inducible gene-I mediates late phase induction of TNF-alpha by lipopolysaccharide.  |  Wang, J., et al. 2008. J Immunol. 180: 8011-9. PMID: 18523264
  11. Recognition of lipopolysaccharide pattern by TLR4 complexes.  |  Park, BS. and Lee, JO. 2013. Exp Mol Med. 45: e66. PMID: 24310172
  12. Transport of lipopolysaccharide to the Gram-negative bacterial cell surface.  |  Putker, F., et al. 2015. FEMS Microbiol Rev. 39: 985-1002. PMID: 26038291
  13. Structure of the O-Antigen and the Lipid A from the Lipopolysaccharide of Fusobacterium nucleatum ATCC 51191.  |  Garcia-Vello, P., et al. 2021. Chembiochem. 22: 1252-1260. PMID: 33197108
  14. Lipopolysaccharide from Gut-Associated Lymphoid-Tissue-Resident Alcaligenes faecalis: Complete Structure Determination and Chemical Synthesis of Its Lipid A.  |  Shimoyama, A., et al. 2021. Angew Chem Int Ed Engl. 60: 10023-10031. PMID: 33522128

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

LPS, 10 mg

sc-3535
10 mg
$110.00

LPS, 25 mg

sc-3535A
25 mg
$230.00

LPS, 100 mg

sc-3535B
100 mg
$415.00

What strain of gram negative bacteria is this isolated from?

Asked by: Dr Buck
Thank you for your question. LPS is derived from Escherichia coli (O111:B4) . The source of the strain is proprietary.
Answered by: BlakeJ
Date published: 2025-01-02

Which species was the LPS derived from?

Asked by: Walid
Thank you for your question. LPS is derived from Escherichia coli (O111:B4) .
Answered by: BlakeJ
Date published: 2024-11-06

You recommend preparing a stock solution by adding 1 mL to 1 mg LPS. The smallest order unit is 10 mg and we are not able to weigh 1 mg. How can we proceed? Thanks you.

Asked by: pS2023
Thank you for your question. The 10 mg unit size of this product can be dissolved in a volume of 10 ml of sterile balanced salt solution or cell culture medium. This stock solution can then be aliquoted and frozen for up to 2 years.
Answered by: Tech Support Europe
Date published: 2023-04-26

On the LPS product, I need to know the species that the LPS was derived from. Thanx Drmajbuck

Asked by: Dr Buck
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-06

What is the physical appearance?

Asked by: SCM4
Previous lots have been faint yellow in color.
Answered by: Tech Service 11
Date published: 2018-08-06
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Rated 5 out of 5 by from LPS induces experimental sepsis and lung injuryWhen administered intraperitoneally, the LPS from Santa Cruz induces consistent levels of lung injury and inflammation in a mouse model of sepsis-induced lung injury, as measured by vascular permeability assays, neutrophil activity assays, inflammatory cytokine expression, and wet:dry weight ratio.
Date published: 2022-05-16
Rated 5 out of 5 by from Very wellWe experimentally determined that many of the cellular signals caused by Gram negative bacteria are due to LPS and have also been observed to activate the TNF receptor signaling pathway.
Date published: 2017-02-09
Rated 5 out of 5 by from lipopolysaccharideWe have determined that lipopolysaccharide is an endotoxin present as a key glycolipid component of the outer cell wall of gram-negative bacteria, although the compound is known, it can be measured as an effective proinflammatory agent, and in several cells Type induces proinflammatory cytokines.
Date published: 2017-02-03
Rated 5 out of 5 by from Arrived quicklyArrived quickly - performed as expected.
Date published: 2015-10-08
Rated 5 out of 5 by from Used and worked wellUsed and worked well.
Date published: 2015-09-09
Rated 5 out of 5 by from Works great in our experimentsWorks great in our experiments, no complaints.
Date published: 2015-03-25
Rated 5 out of 5 by from An increase in cleaved caspaseAn increase in cleaved caspase-1 (p10) was observed by WB in THP-1 cells post treatment with LPS. -SCBT QC
Date published: 2015-02-01
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LPS is rated 5.0 out of 5 by 7.
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