LONP2 Double Nickase Plasmid (h): sc-413504-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LONP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LONP2 Double Nickase Plasmid (h) and LONP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LONP2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LONP2 Double Nickase Plasmid (h) | sc-413504-NIC | 20 µg | $410.00 | |||
LONP2 Double Nickase Plasmid (h2) | sc-413504-NIC-2 | 20 µg | $410.00 |
LONP2 encodes a peroxisomal Lon family AAA+ ATP-dependent protease that contributes to peroxisome quality control by recognizing, unfolding, and degrading damaged or misfolded matrix proteins. By maintaining peroxisomal proteostasis, LONP2 supports lipid metabolic processes including fatty acid β-oxidation and reactive oxygen species management that intersect with broader cellular stress responses. Altered peroxisome function and oxidative imbalance are implicated in metabolic and neurodegenerative phenotypes, making LONP2 a useful node for studying pathways linking organelle maintenance to cellular homeostasis. Human LONP2 is therefore relevant to investigations of peroxisome-associated metabolism, protein turnover, and stress adaptation.
LONP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LONP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LONP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LONP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LONP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.