LOC729991-MEF2B Double Nickase Plasmid (h): sc-405289-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC729991-MEF2B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOC729991-MEF2B Double Nickase Plasmid (h) and LOC729991-MEF2B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BORCS8-MEF2B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC729991-MEF2B Double Nickase Plasmid (h) | sc-405289-NIC | 20 µg | $410.00 | |||
LOC729991-MEF2B Double Nickase Plasmid (h2) | sc-405289-NIC-2 | 20 µg | $410.00 |
BORCS8-MEF2B encodes a chimeric BORCS8–MEF2B protein product (LOC729991-MEF2B) that links a component of the BORC complex, which regulates lysosome positioning and kinesin-driven transport, with MEF2B, a MADS-box transcription factor involved in activity-dependent gene regulation. MEF2 family signaling integrates calcium/MAPK inputs to control transcriptional programs governing proliferation, differentiation, and stress responses, while BORC-associated trafficking impacts autophagy and endolysosomal homeostasis. Dysregulation of MEF2B has been associated with altered transcriptional control in lymphoid biology, including recurrent genomic alterations reported in B-cell malignancies, making this locus relevant for dissecting transcription–trafficking crosstalk in disease-relevant cellular contexts.
LOC729991-MEF2B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BORCS8-MEF2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BORCS8-MEF2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BORCS8-MEF2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BORCS8-MEF2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.