LOC285636 Double Nickase Plasmid (h): sc-415435-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC285636 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOC285636 Double Nickase Plasmid (h) and LOC285636 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting C5orf51. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC285636 Double Nickase Plasmid (h) | sc-415435-NIC | 20 µg | $410.00 | |||
LOC285636 Double Nickase Plasmid (h2) | sc-415435-NIC-2 | 20 µg | $410.00 |
C5orf51 encodes the human protein LOC285636, an incompletely characterized gene product with limited functional annotation in current pathway databases. Available genomic and transcriptomic evidence suggests context-dependent expression, supporting investigation of potential roles in gene regulatory networks, cell-state maintenance, and lineage-specific programs. Because poorly characterized open reading frame loci can influence RNA processing, chromatin-associated regulation, or proteostasis indirectly, perturbation studies are commonly used to connect LOC285636 to defined cellular processes. Profiling LOC285636 disruption across relevant model systems can help associate this locus with molecular signatures observed in disease-relevant phenotypes without implying clinical outcomes.
LOC285636 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the C5orf51 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within C5orf51. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt C5orf51 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of C5orf51-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.