LOC147710 Double Nickase Plasmid (h): sc-418022-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC147710 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LOC147710 Double Nickase Plasmid (h) and LOC147710 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IGSF23. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC147710 Double Nickase Plasmid (h) | sc-418022-NIC | 20 µg | $410.00 | |||
LOC147710 Double Nickase Plasmid (h2) | sc-418022-NIC-2 | 20 µg | $410.00 |
IGSF23 (also referred to as LOC147710) encodes an immunoglobulin superfamily protein predicted to participate in cell–cell communication and adhesion-like processes at the plasma membrane. As an Ig-domain–containing factor, it is relevant for investigating how surface receptors and scaffolding proteins coordinate cytoskeletal organization, migration, and tissue-specific signaling. Although functional annotation remains limited, expression and genetic perturbation studies of IGSF23 can help define roles in immune-related communication, epithelial organization, and context-dependent signaling networks. Dysregulation of Ig superfamily members is frequently explored in inflammation and oncology, making IGSF23 a useful target for mechanistic studies of cell interaction phenotypes and pathway connectivity.
LOC147710 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IGSF23 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IGSF23. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IGSF23 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IGSF23-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.