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Lithium β-hydroxypyruvate (CAS 3369-79-7)

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Alternate Names:
hydroxypyruvic acid
Application:
Lithium β-hydroxypyruvate is a substrate for transketolase
CAS Number:
3369-79-7
Purity:
>97%
Molecular Weight:
109.99
Molecular Formula:
C3H3LiO4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Lithium β-hydroxypyruvate is a substrate for transketolase, and used for the production of optically pure L-2-hydroxy aldehydes.


Lithium β-hydroxypyruvate (CAS 3369-79-7) References

  1. Enzymatic and mechanistic studies on the formation of N-phenylglycolohydroxamic acid from nitrosobenzene and pyruvate in spinach leaf homogenate.  |  Tatsunami, R. and Yoshioka, T. 2006. J Agric Food Chem. 54: 590-6. PMID: 16417326
  2. Purification and characterization of hydroxypyruvate reductase from the facultative methylotroph Methylobacterium extorquens AM1.  |  Chistoserdova, LV. and Lidstrom, ME. 1991. J Bacteriol. 173: 7228-32. PMID: 1657886
  3. Purification and characterization of hydroxypyruvate reductase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2.  |  Izumi, Y., et al. 1990. Eur J Biochem. 190: 279-84. PMID: 2114287
  4. Identification of catalytically important amino acid residues for enzymatic reduction of glyoxylate in plants.  |  Hoover, GJ., et al. 2013. Biochim Biophys Acta. 1834: 2663-71. PMID: 24076009
  5. An efficient amperometric transketolase assay: towards inhibitor screening.  |  Touisni, N., et al. 2014. Biosens Bioelectron. 62: 90-6. PMID: 24984289
  6. Real-time pH monitoring of industrially relevant enzymatic reactions in a microfluidic side-entry reactor (μSER) shows potential for pH control.  |  Gruber, P., et al. 2017. Biotechnol J. 12: PMID: 28106351
  7. Enzymatic synthesis of chiral amino-alcohols by coupling transketolase and transaminase-catalyzed reactions in a cascading continuous-flow microreactor system.  |  Gruber, P., et al. 2018. Biotechnol Bioeng. 115: 586-596. PMID: 28986983
  8. A catechol oxidase AcPPO from cherimoya (Annona cherimola Mill.) is localized to the Golgi apparatus.  |  Olmedo, P., et al. 2018. Plant Sci. 266: 46-54. PMID: 29241566
  9. One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose.  |  Bawn, M., et al. 2018. Enzyme Microb Technol. 116: 16-22. PMID: 29887012
  10. Substrate specificity and carbohydrate synthesis using transketolase  |  Yoshihiro Kobori, David C. Myles, and George M. Whitesides. 1992. J. Org. Chem. 58: 5899-5907.
  11. Preparation of optically pure L-2-hydroxyaldehydes with yeast transketolase  |  Franz Effenberger ∗, Volker Null, Thomas Ziegler. 1992. Tetrahedron Letters. 33: 5157-5160.
  12. Chiral polyol synthesis catalyzed by a thermostable Transketolase immobilized on Layered Double Hydroxides in Ionic liquids  |  Dr. Ghina Ali, Dr. Thomas Moreau, Prof. Claude Forano, Dr. Christine Mousty, Dr. Vanessa Prevot, Dr. Franck Charmantray, Prof. Laurence Hecquet. 2015. ChemCatChem. 7: 3163-3170.
  13. Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose  |  Fabiana Subrizi a, Max Cárdenas-Fernández b, Gary J. Lye b, John M. Ward b, Paul A. Dalby b, Tom D. Sheppard a and Helen C. Hailes *a. 2016. Green chemistry. 18: 3158-3165.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Lithium β-hydroxypyruvate, 1 g

sc-252955
1 g
$292.00

sc-252955 is a lithium salt, rather than a sodium salt, is it appropriate for use in the enzyme assay for hydroxypyruvate reductase? I am concerned as to whether the lithium could interact.

Asked by: ChemSynth123
Thank you for your question. Lithium β-hydroxypyruvate (CAS 3369-79-7): sc-252955 is appropriate for used as a standard assay mixture for hydroxypyruvate reductase.
Answered by: Chemical Support 7
Date published: 2017-03-07
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Rated 5 out of 5 by from YiYi, D. et al. (PubMed 23001740) developed a pH-based high-throughput assay method for the rapid and reliable measurement of transketolase activity. The method is based on the decarboxylation of Lithium -hydroxypyruvate as a hydroxyacetyl donor with an aldehyde acceptor, using phenol red as the pH indicator. Upon release of carbon dioxide from Lithium -hydroxypyruvate, the pH increase in the reaction mixture can be determined photometrically by the color change of the pH indicator. -SCBT Publication Review
Date published: 2015-07-08
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Lithium β-hydroxypyruvate is rated 5.0 out of 5 by 1.
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