LHX3 Antibody (2C10): sc-293411
- LHX3 Antibody (2C10) is a mouse monoclonal IgG2a κ LHX3 antibody provided at 100 µg/ml
- raised against amino acids 228-316 of LHX3 of human origin
- recommended for detection of LHX3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LHX3 Antibody (2C10). This work is in progress.
LHX3 Antibody (2C10) is a mouse monoclonal IgG2a kappa light chain antibody that detects LHX3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). LHX3 (2C10) antibody is available as the non-conjugated anti-LHX3 monoclonal isotype antibody. LHX3 protein plays a crucial role in the development of the central nervous system, particularly in the specification of motor neurons and the formation of the pituitary gland. LHX3 is essential for regulating cell migration and axon navigation during neuronal development. LHX3 exists in two isoforms, LHX3a and LHX3b, which differ at the amino terminus but share conserved LIM domains and a homeodomain that is vital for function. The homeodomain contains three nuclear localization signals, allowing LHX3 to effectively target the nucleus, where LHX3 exerts transcriptional regulatory functions. Both isoforms are predominantly expressed in the adult pituitary gland, spinal cord, and lungs, highlighting their importance in these tissues. The short LHX3b isoform has been shown to inhibit DNA binding and transcriptional activity, indicating a complex regulatory mechanism. Human LHX3 is located at the subtelomeric region of chromosome 9 at band 9q34.3, a site associated with chromosomal translocations and insertions, suggesting involvement in various developmental disorders of the central nervous system.
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LHX3 Antibody (2C10) References:
- Differential activation of pituitary hormone genes by human Lhx3 isoforms with distinct DNA binding properties. | Sloop, KW., et al. 1999. Mol Endocrinol. 13: 2212-25. PMID: 10598593
- Analysis of the human LHX3 neuroendocrine transcription factor gene and mapping to the subtelomeric region of chromosome 9. | Sloop, KW., et al. 2000. Gene. 245: 237-43. PMID: 10717474
- The homeodomain coordinates nuclear entry of the Lhx3 neuroendocrine transcription factor and association with the nuclear matrix. | Parker, GE., et al. 2000. J Biol Chem. 275: 23891-8. PMID: 10818088
- Genomic structure, chromosomal localization, and expression pattern of the human LIM-homeobox3 (LHX 3) gene. | Schmitt, S., et al. 2000. Biochem Biophys Res Commun. 274: 49-56. PMID: 10903894
- Genetic and epigenetic mechanisms contribute to motor neuron pathfinding. | Sharma, K., et al. 2000. Nature. 406: 515-9. PMID: 10952312
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX3 Antibody (2C10) | sc-293411 | 100 µg/ml | $316.00 |