Lewis X Trisaccharide 4-Methylumbelliferyl Glycoside Nonaacetate

Lewis X Trisaccharide 4-Methylumbelliferyl Glycoside Nonaacetate is a carbohydrate derivative containing a Lewis X trisaccharide linked to a fluorescent 4-methylumbelliferyl group, which allows researchers to study glycosylation pathways and enzyme activities associated with Lewis X antigens. The Lewis X structure is a fucosylated trisaccharide that plays a role in cell adhesion, immune recognition, and pathogen-host interactions. Its nonacetylated form allows the molecule to remain protected and stable, providing an ideal substrate for detailed analysis. In research applications, this compound is valuable for investigating the specificity of glycosyltransferases and glycosidases that modify the Lewis X structure. The 4-methylumbelliferyl group serves as a fluorescent marker, enabling sensitive detection and quantification of enzymatic activities. This property is exploited in enzyme assays, where researchers can measure enzyme kinetics and substrate preferences with precision. The compound also facilitates the study of carbohydrate-binding proteins such as lectins, which interact specifically with the Lewis X trisaccharide and are involved in immune responses and pathogen recognition. The nonacetylated glycoside derivative aids in synthesizing glycan structures that mimic natural antigens, providing tools to probe glycan-protein interactions and explain mechanisms underlying cell recognition and adhesion. This makes it a versatile tool for studying glycosylation processes and the role of Lewis X in glycobiology.