Leptomycin B, Free Acid (CAS 87081-35-4)
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Leptomycin B, Free Acid, a cytotixin and Streptomyces metabolite, may act to be a specific inhibitor of nuclear export signal-dependent transport via inhibition of CRM1. CRM1 is a protein required for nuclear export of proteins containing a nuclear export sequence (NES). Leptomycin B has been reported to block Rev-dependent export of mRNA into the cytoplasm, which inhibits Rev-dependent gene expression. Leptomycin B has also displayed a capacity to inhibit translation and export of many RNAs, including c-Fos and Cox-2 mRNAs, by inhibiting export of ribonucleoproteins. Additional experiments have shown leptomycin B to be an antibiotic which induces cell elongation and suppression of fission yeast. Furthermore, proliferation of rat fibroblasts was shown to be reversibly suppressed by leptomycin B in both the G1 and G2 phases, forcing the cells into a resting state (GO).
Leptomycin B, Free Acid (CAS 87081-35-4) References
- Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. | Kudo, N., et al. 1999. Proc Natl Acad Sci U S A. 96: 9112-7. PMID: 10430904
- Nuclear export of MAP kinase (ERK) involves a MAP kinase kinase (MEK)-dependent active transport mechanism. | Adachi, M., et al. 2000. J Cell Biol. 148: 849-56. PMID: 10704436
- The transcriptional activator Mirk/Dyrk1B is sequestered by p38alpha/beta MAP kinase. | Lim, S., et al. 2002. J Biol Chem. 277: 49438-45. PMID: 12384504
- Exportin 1-independent nuclear export of GAPDH. | Schmitz, HD., et al. 2003. Cell Biol Int. 27: 511-7. PMID: 12842090
- Phosphorylation of clock protein PER1 regulates its circadian degradation in normal human fibroblasts. | Miyazaki, K., et al. 2004. Biochem J. 380: 95-103. PMID: 14750904
- Uncapped mRNA introduced into tobacco protoplasts can be imported into the nucleus and is trapped by leptomycin B. | Stuger, R. and Forreiter, C. 2004. Plant Cell Rep. 23: 99-103. PMID: 15221275
- Phosphorylation-regulated nucleocytoplasmic trafficking of internalized fibroblast growth factor-1. | Wiedłocha, A., et al. 2005. Mol Biol Cell. 16: 794-810. PMID: 15574884
- A regulated nucleocytoplasmic shuttle contributes to Bright's function as a transcriptional activator of immunoglobulin genes. | Kim, D. and Tucker, PW. 2006. Mol Cell Biol. 26: 2187-201. PMID: 16507996
- Proteasome-dependent down-regulation of activated Stat5A in the nucleus. | Chen, Y., et al. 2006. Blood. 108: 566-74. PMID: 16569768
- Effects of leptomycin B on the cell cycle of fibroblasts and fission yeast cells. | Yoshida, M., et al. 1990. Exp Cell Res. 187: 150-6. PMID: 2298254
- Epigallocatechin-3-gallate enhances the therapeutic effects of leptomycin B on human lung cancer a549 cells. | Cromie, MM. and Gao, W. 2015. Oxid Med Cell Longev. 2015: 217304. PMID: 25922640
- Lifetime-based photoconversion of EGFP as a tool for FLIM. | Herman, P., et al. 2019. Biochim Biophys Acta Gen Subj. 1863: 266-277. PMID: 30394285
- Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA. | Wolff, B., et al. 1997. Chem Biol. 4: 139-47. PMID: 9190288
- CRM1 is responsible for intracellular transport mediated by the nuclear export signal. | Fukuda, M., et al. 1997. Nature. 390: 308-11. PMID: 9384386
- Discrimination between NL1- and NL2-mediated nuclear localization of the glucocorticoid receptor. | Savory, JG., et al. 1999. Mol Cell Biol. 19: 1025-37. PMID: 9891038
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Leptomycin B, Free Acid, 100 µg | sc-202210 | 100 µg | $196.00 | |||
Leptomycin B, Free Acid, 500 µg | sc-202210A | 500 µg | $689.00 |