LEPREL4 Antibody (F-7): sc-514276
- LEPREL4 Antibody (F-7) is a mouse monoclonal IgG3 κ LEPREL4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 190-210 within an internal region of LEPREL4 of human origin
- recommended for detection of LEPREL4 of human and mouse origin and Sc65 of rat origin by WB, IP, IF and ELISA
- Anti-LEPREL4 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG3 BP-HRP is the preferred secondary detection reagent for LEPREL4 Antibody (F-7) for WB applications. This reagent is now offered in a bundle with LEPREL4 Antibody (F-7) (see ordering information below).
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LEPREL4 Antibody (F-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects LEPREL4 protein of human, mouse, and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LEPREL4 Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LEPREL4, also known as SC65 or NOL55, is a 437 amino acid member of the leprecan family, primarily located in the granular component of the nucleolus and on the surface of chromosomes during mitosis. This localization suggests a role in chromosomal organization and stability, which is vital for proper cell division and genetic integrity. LEPREL4 undergoes post-translational modification by phosphorylation at tyrosine 234, a modification that can influence function and interactions with other proteins. LEPREL4 gene is situated on human chromosome 17, a region with high gene density that houses critical tumor suppressor genes such as p53 and BRCA1, which are essential for maintaining cellular integrity and preventing malignant transformations.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LEPREL4 Antibody (F-7) References:
- p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis. | Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
- Distinct pattern of p53 phosphorylation in human tumors. | Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
- Three primary malignancies related to BRCA mutation successively occurring in a BRCA1 185delAG mutation carrier. | Piura, B., et al. 2001. Eur J Obstet Gynecol Reprod Biol. 97: 241-4. PMID: 11451557
- Closing in on a breast cancer gene on chromosome 17q. | Hall, JM., et al. 1992. Am J Hum Genet. 50: 1235-42. PMID: 1598904
- Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling. | Wang, Y., et al. 2006. EMBO J. 25: 5058-70. PMID: 17053785
- cDNA cloning and characterization of a novel nucleolar protein. | Ochs, RL., et al. 1996. Mol Biol Cell. 7: 1015-24. PMID: 8862517
- A detailed study of loss of heterozygosity on chromosome 17 in tumours from Li-Fraumeni patients carrying a mutation to the TP53 gene. | Varley, JM., et al. 1997. Oncogene. 14: 865-71. PMID: 9047394
- The Li-Fraumeni syndrome: an inherited susceptibility to cancer. | Evans, SC. and Lozano, G. 1997. Mol Med Today. 3: 390-5. PMID: 9302689
- Loss of heterozygosity for defined regions on chromosomes 3, 11 and 17 in carcinomas of the uterine cervix. | Kersemaekers, AM., et al. 1998. Br J Cancer. 77: 192-200. PMID: 9460988
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LEPREL4 Antibody (F-7) | sc-514276 | 200 µg/ml | $322.00 | |||
LEPREL4 Antibody (F-7): m-IgG3 BP-HRP Bundle | sc-550609 | 200 µg Ab; 40 µg BP | $361.00 | |||
LEPREL4 Antibody (F-7) AC | sc-514276 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LEPREL4 Antibody (F-7) HRP | sc-514276 HRP | 200 µg/ml | $322.00 | |||
LEPREL4 Antibody (F-7) FITC | sc-514276 FITC | 200 µg/ml | $336.00 | |||
LEPREL4 Antibody (F-7) PE | sc-514276 PE | 200 µg/ml | $349.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 488 | sc-514276 AF488 | 200 µg/ml | $364.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 546 | sc-514276 AF546 | 200 µg/ml | $364.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 594 | sc-514276 AF594 | 200 µg/ml | $364.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 647 | sc-514276 AF647 | 200 µg/ml | $364.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 680 | sc-514276 AF680 | 200 µg/ml | $364.00 | |||
LEPREL4 Antibody (F-7) Alexa Fluor® 790 | sc-514276 AF790 | 200 µg/ml | $364.00 | |||
LEPREL4 (F-7) Neutralizing Peptide | sc-514276 P | 100 µg/0.5 ml | $69.00 |