Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Legumain Double Nickase Plasmid (h): sc-402437-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Legumain Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Legumain Double Nickase Plasmid (h) and Legumain Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LGMN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Legumain Antibody (B-8): sc-133234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Legumain Double Nickase Plasmid (h) | sc-402437-NIC | 20 µg | $410.00 | |||
Legumain Double Nickase Plasmid (h2) | sc-402437-NIC-2 | 20 µg | $410.00 |
LGMN encodes legumain, a lysosomal asparaginyl endopeptidase that contributes to proteolytic processing of endocytosed proteins and antigen presentation through MHC class II pathways. By shaping lysosome function and extracellular matrix remodeling via protease networks, legumain influences immune regulation, tissue turnover, and stress responses linked to endo-lysosomal trafficking. Dysregulated LGMN expression or activity has been associated with tumor microenvironment biology, inflammatory conditions, and neurodegenerative processes where altered proteostasis and lysosomal pathways are prominent.
Legumain Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LGMN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LGMN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LGMN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LGMN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.