LARP1 Double Nickase Plasmid (h): sc-404097-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LARP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LARP1 Double Nickase Plasmid (h) and LARP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LARP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LARP1 Antibody (A-8): sc-515873
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LARP1 Double Nickase Plasmid (h) | sc-404097-NIC | 20 µg | $410.00 |
LARP1 (La-related protein 1) is an RNA-binding protein that couples mRNA fate to nutrient and growth signals, with prominent roles in translational control and mRNA stability. It interacts with the mTORC1 pathway and regulates translation of TOP mRNAs that encode ribosomal proteins and translation factors, linking ribosome biogenesis to cellular proliferation and stress responses. Through modulation of cap-dependent translation, LARP1 contributes to cell cycle progression and adaptive programs such as autophagy and metabolic remodeling. Dysregulated LARP1 expression or signaling context has been associated with proliferative phenotypes and altered translational landscapes in diverse disease models, supporting its use as a node for studying growth control.
LARP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LARP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LARP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LARP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LARP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.