LAP2 Antibody (3A3): sc-81610

LAP2 Antibody (3A3) is a mouse monoclonal IgG1 kappa light chain antibody that detects LAP2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-LAP2 antibody (3A3) is available as the non-conjugated format. LAP2, also known as thymopoietin, plays a crucial role in maintaining the structural integrity of the nuclear envelope, which is essential for proper cellular function. Located within the nuclear envelope, LAP2 interacts with chromatin and the nuclear lamina, contributing to the organization and stability of the nucleus. LAP2 features a unique amino-terminal domain that allows DNA binding, and contains the LAP2-emerin-MAN1 (LEM) motif, which is important for interactions with other nuclear proteins. Alternative splicing of LAP2 results in several isoforms, including LAP2α and LAP2β, which have distinct functions; LAP2α associates with the internal nucleoskeleton and binds to lamin A, while LAP2β interacts with lamin B and chromosomal barrier-to-autointegration factor (BAF), suggesting a role in chromatin stabilization. The gene encoding human LAP2 is located on chromosome 12q23.1, highlighting its significance in genomic organization and cellular architecture.

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LAP2 Antibody (3A3) References:

1. MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin.  |  Lin, F., et al. 2000. J Biol Chem. 275: 4840-7. PMID: 10671519
2. Review: lamina-associated polypeptide 2 isoforms and related proteins in cell cycle-dependent nuclear structure dynamics.  |  Dechat, T., et al. 2000. J Struct Biol. 129: 335-45. PMID: 10806084
3. Lamina-associated polypeptide 2alpha binds intranuclear A-type lamins.  |  Dechat, T., et al. 2000. J Cell Sci. 113 Pt 19: 3473-84. PMID: 10984438
4. LAP2 binds to BAF.DNA complexes: requirement for the LEM domain and modulation by variable regions.  |  Shumaker, DK., et al. 2001. EMBO J. 20: 1754-64. PMID: 11285238
5. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA.  |  Cai, M., et al. 2001. EMBO J. 20: 4399-407. PMID: 11500367
6. Loss of LAP2 alpha delays satellite cell differentiation and affects postnatal fiber-type determination.  |  Gotic, I., et al. 2010. Stem Cells. 28: 480-8. PMID: 20039368
7. Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells.  |  Li, J., et al. 2014. Biochem Biophys Res Commun. 446: 267-71. PMID: 24589738
8. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription.  |  Mirza, AN., et al. 2019. Cell. 176: 198-212.e15. PMID: 30503211
9. Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity.  |  Maurizi, E., et al. 2022. Int J Mol Sci. 23: PMID: 35628669
10. Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF.  |  Keller, D., et al. 2024. iScience. 27: 109343. PMID: 38510147
11. Structure and mapping of the human thymopoietin (TMPO) gene and relationship of human TMPO beta to rat lamin-associated polypeptide 2.  |  Harris, CA., et al. 1995. Genomics. 28: 198-205. PMID: 8530026
12. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.  |  Yang, L., et al. 1997. J Cell Biol. 139: 1077-87. PMID: 9382857