Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Langerin CRISPR Activation Plasmid (h): sc-401949-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Langerin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Langerin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Langerin CRISPR Activation Plasmid (h) and Langerin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the CD207 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Langerin Antibody (H-4): sc-271272
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Langerin CRISPR Activation Plasmid (h) | sc-401949-ACT | 20 µg | $397.00 |
CD207 encodes Langerin, a C-type lectin receptor enriched in Langerhans cells and related dendritic cell subsets that functions in pathogen recognition and antigen capture at epithelial barriers. Langerin binds carbohydrate motifs in a Ca²⁺-dependent manner and mediates endocytosis into Birbeck granules, shaping antigen processing and presentation programs that influence adaptive immune priming. Through lectin-driven uptake and routing to endosomal compartments, it intersects with innate immune sensing and inflammatory signaling that modulate cytokine outputs and T cell polarization. Altered CD207/Langerin expression or Langerhans cell function is relevant to studies of skin immune homeostasis, allergic inflammation, and infection-associated barrier immune responses.
Langerin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous CD207 expression without altering the underlying DNA sequence.
Langerin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the CD207 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the CD207 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Langerin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native CD207 locus and enabling the study of Langerin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Langerin pathway restoration in tumor cells with silenced or reduced CD207 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.