LAMP-2 Double Nickase Plasmid (m): sc-421383-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LAMP-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LAMP-2 Double Nickase Plasmid (m) and LAMP-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Lamp2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LAMP-2 Antibody (ABL-93): sc-20004
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAMP-2 Double Nickase Plasmid (m) | sc-421383-NIC | 20 µg | $410.00 | |||
LAMP-2 Double Nickase Plasmid (m2) | sc-421383-NIC-2 | 20 µg | $410.00 |
Mouse Lamp2 encodes lysosome-associated membrane protein 2 (LAMP-2), an abundant glycoprotein of the lysosomal limiting membrane that supports lysosome integrity, vesicle trafficking, and degradative capacity. LAMP-2 participates in autophagy-related pathways, including chaperone-mediated autophagy and autophagosome–lysosome fusion, thereby influencing turnover of long-lived proteins and organelles. Disruption of LAMP-2 function is linked to impaired lysosomal homeostasis, altered metabolic stress responses, and accumulation of autophagic substrates, processes relevant to cardiomyopathy and neurodegeneration models. Lamp2 is therefore widely studied in contexts involving endolysosomal trafficking, proteostasis, and inflammatory signaling driven by defective cellular clearance.
LAMP-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Lamp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Lamp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Lamp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Lamp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.