Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Laminin β-1/γ-1 Antibody (612C10) is a high quality monoclonal Laminin beta-1/ gamma-1 antibody (also designated Laminin β-1/γ-1 antibody) suitable for the detection of the Laminin beta-1/ gamma-1 protein of human origin. Laminin β-1/γ-1 Antibody (612C10) is available as the non-conjugated anti-Laminin beta-1/ gamma-1 antibody. Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each laminin is a heterotrimer of α, β and γ chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan and cognate laminin receptors. The human Laminin β-1 gene maps to chromosome 7q22; the human Laminin γ-1 gene maps to chromosome 1q31. Both are ubiquitously expressed in tissues that produce basement membranes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using Laminin β-1/γ-1 (612C10): sc-47766 antibody for immunoprecipitation, how much antibody should I use?
Asked by: Professor Griffin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: