Lamin A Antibody (4A58): sc-71481

Lamin A Antibody (4A58) is a mouse monoclonal IgG3 antibody designed to detect Lamin A, also known as LMNA or A-type lamin, across multiple species including mouse, rat, human, bovine, and canine. Lamin A is a crucial component of the nuclear lamina, a dense fibrillar network that provides structural support to the nuclear envelope, ensuring nuclear integrity and maintaining the proper organization of chromatin. This protein features a characteristic tripartite structure with a central alpha-helical rod domain flanked by globular head and tail domains, which facilitate the formation of a stable intermediate filament network essential for cellular stability and gene regulation. The structural integrity provided by Lamin A is vital for various cellular processes, including DNA replication, transcription, and the regulation of the cell cycle, making Lamin A indispensable for maintaining cellular longevity and function. Lamin A is processed from its precursor, prelamin A, through a series of post-translational modifications, highlighting Lamin A′s dynamic role in nuclear architecture. Anti-Lamin A antibody (4A58) is raised against a partially purified recombinant human Lamin A protein, and does not cross-react with Lamin C, a closely related splice variant. Lamin A (4A58) antibody is versatile for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications, providing reliable and consistent results for researchers studying nuclear structure, gene expression regulation, and laminopathies. Anti-Lamin A antibody (4A58) facilitates advancements in understanding the molecular mechanisms underlying various diseases linked to LMNA mutations.

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Lamin A Antibody (4A58) References:

1. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.  |  McKeon, FD., et al. Nature. 319: 463-8. PMID: 3453101
2. cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.  |  Fisher, DZ., et al. 1986. Proc Natl Acad Sci U S A. 83: 6450-4. PMID: 3462705
3. The dynamic properties and possible functions of nuclear lamins.  |  Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
4. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
5. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
6. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
7. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
8. Lamin proteolysis facilitates nuclear events during apoptosis.  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Lamin A Antibody (4A58) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-Lamin A Antibody (4A58) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Lamin A Antibody (4A58) Specifications

Alternate Product Name: Lamin A

Product Clone Name: 4A58

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids:

Epitope Target: Lamin A

Epitope Species: human

Product Reactivity/Detection: Lamin A

Species Reactivity/Detection: mouse, rat, human, bovine, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 50 µg IgG₃ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for Lamin A Antibody (4A58): N/A