Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-type Ca++ CP α1D CRISPR/Cas9 KO Plasmid (h): sc-401745
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-type Ca++ CP α1D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-type Ca++ CP α1D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-type Ca++ CP α1D Antibody (E-3): sc-515679
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-type Ca++ CP α1D CRISPR/Cas9 KO Plasmid (h) | sc-401745 | 20 µg | $397.00 |
Overview
CACNA1D encodes the pore-forming α1D subunit (CaV1.3) of L-type voltage-gated calcium channels that couple membrane depolarization to Ca2+ influx and downstream Ca2+-dependent signaling. CaV1.3 contributes to excitation–transcription coupling by shaping cytosolic Ca2+ dynamics that regulate calcium/calmodulin pathways, kinase cascades, and activity-dependent gene expression programs. In non-excitable and excitable cells, CACNA1D activity influences cellular differentiation, secretion, and electrophysiological properties through modulation of calcium homeostasis. Genetic and regulatory alterations in CACNA1D have been associated with disorders involving aberrant electrical signaling and calcium-dependent transcriptional control, supporting mechanistic studies of channel function in disease-relevant cellular models.
L-type Ca++ CP α1D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CACNA1D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CACNA1D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CACNA1D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-type Ca++ CP α1D protein expression.
This CRISPR knockout system enables efficient generation of CACNA1D-deficient cell models for investigation of L-type Ca++ CP α1D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CACNA1D exon(s) critical for L-type Ca++ CP α1D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CACNA1D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-type Ca++ CP α1D CRISPR/Cas9 KO Plasmid (h) and L-type Ca++ CP α1D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CACNA1D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-type Ca++ CP α1D HDR Plasmid (h) and L-type Ca++ CP α1D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CACNA1D homology arms to support homology-directed repair at defined CACNA1D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.