Molecular structure of L-Histidine 4-nitroanilide, CAS Number: 70324-65-1
L-Histidine 4-nitroanilide (CAS 70324-65-1)
Alternate Names:
L-Histidinep-nitroanilide; H-His-pNA
CAS Number:
70324-65-1
Molecular Weight:
275.26
Molecular Formula:
C12H13N5O3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
L-Histidine 4-nitroanilide serves as a significant chemical compound in biochemical research, employed as a substrate to measure the activity of enzymes like chymotrypsin, trypsin, and elastase. Its synthesis occurs through the reaction between L-histidine and 4-nitroaniline, leading to a product displaying a yellow color. The mode of action for L-Histidine 4-nitroanilide involves enzyme-catalyzed hydrolysis of the amide bond connecting L-histidine and 4-nitroaniline. Consequently, this hydrolysis causes the release of 4-nitroaniline, which can be detected spectrophotometrically at 410 nm. Throughout biochemical research, this compound finds broad application due to its utility in enzyme activity measurement.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-Histidine 4-nitroanilide, 250 mg | sc-295286 | 250 mg | $159.00 | |||
L-Histidine 4-nitroanilide, 1 g | sc-295286A | 1 g | $477.00 |