L-ficolin Double Nickase Plasmid (h2): sc-404837-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-ficolin Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- L-ficolin Double Nickase Plasmid (h2) and L-ficolin Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting FCN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-ficolin Antibody (FCN219): sc-130297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-ficolin Double Nickase Plasmid (h2) | sc-404837-NIC-2 | 20 µg | $410.00 |
Human FCN2 encodes L-ficolin, a soluble pattern-recognition lectin primarily produced by the liver that initiates the lectin pathway of complement by binding microbial and altered-self carbohydrate motifs and recruiting MASP proteases to drive C4/C2 activation, opsonization, and inflammatory signaling. Through modulation of complement-dependent clearance, agglutination, and phagocyte engagement, L-ficolin contributes to innate immune homeostasis at the interface of infection and sterile inflammation. Genetic variation or dysregulated FCN2 expression has been associated with altered susceptibility to infectious disease and inflammatory phenotypes, supporting its use as a molecular node linking complement activity, hepatic acute-phase responses, and host–pathogen interactions. FCN2 gene editing or perturbation models enable mechanistic studies of lectin-complement activation, glycan-dependent recognition, and downstream immune effector pathways in relevant cellular and in vitro systems.
L-ficolin Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the FCN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FCN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FCN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FCN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.