Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-FABP Double Nickase Plasmid (h): sc-401554-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-FABP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- L-FABP Double Nickase Plasmid (h) and L-FABP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FABP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-FABP Antibody (F-9): sc-271591
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-FABP Double Nickase Plasmid (h) | sc-401554-NIC | 20 µg | $410.00 | |||
L-FABP Double Nickase Plasmid (h2) | sc-401554-NIC-2 | 20 µg | $410.00 |
FABP1 encodes liver fatty acid–binding protein (L-FABP), a high-abundance cytosolic lipid chaperone that binds long-chain fatty acids, acyl-CoAs, bile acids, and other hydrophobic ligands to coordinate intracellular trafficking and metabolism. In hepatocytes and enterocytes, L-FABP supports fatty acid uptake, β-oxidation, triglyceride handling, and lipid-mediated transcriptional programs linked to PPAR signaling and oxidative stress responses. By influencing lipid flux and inflammatory signaling, FABP1 activity intersects with metabolic homeostasis and cellular redox balance. Dysregulation of FABP1 expression or function has been associated with altered hepatic lipid accumulation and susceptibility to metabolic liver phenotypes, making it a useful target for mechanistic studies of lipid-driven pathology.
L-FABP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FABP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FABP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FABP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FABP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.