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L-Alanine 4-nitroanilide (CAS 1668-13-9)

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Alternate Names:
L-Ala-pNA
CAS Number:
1668-13-9
Molecular Weight:
209.2
Molecular Formula:
C9H11N3O3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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L-Alanine-4-nitroanilide serves as a substrate uniquely tailored for the enzyme trypsin, a fundamental component of protease assays. Through its catalytic action, trypsin effectively severs the peptide bond connecting L-alanine and 4-nitroaniline, consequently liberating the distinctive yellow 4-nitroaniline product. Notably, the quantity of generated 4-nitroaniline stands in direct proportion to the level of trypsin activity, and this correlation can be precisely quantified using spectrophotometric measurements.


L-Alanine 4-nitroanilide (CAS 1668-13-9) References

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  2. The effect of cations and complexing agents on the hydrolysis of L-Alanine-4-nitroanilide by aminopeptidase M.  |  Femfert, U. and Pfleiderer, G. 1971. FEBS Lett. 14: 89-91. PMID: 11945726
  3. Aminopeptidases in Caenorhabditis elegans and Panagrellus redivivus: detection using peptide and non-peptide substrates.  |  Masler, EP. 2002. J Helminthol. 76: 45-52. PMID: 12018195
  4. Characterization of Aminopeptidase in the Free-living Nematode Panagrellus redivivus: Subcellular Distribution and Possible Role in Neuropeptide Metabolism.  |  Masler, EP. 2007. J Nematol. 39: 153-60. PMID: 19259484
  5. The structure of Mlc titration factor A (MtfA/YeeI) reveals a prototypical zinc metallopeptidase related to anthrax lethal factor.  |  Xu, Q., et al. 2012. J Bacteriol. 194: 2987-99. PMID: 22467785
  6. Rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria.  |  Manafi, M. and Kneifel, W. 1990. J Appl Bacteriol. 69: 822-7. PMID: 2286581
  7. Reconstitution of purified dipeptidyl peptidase IV. A comparison with aminopeptidase N with respect to morphology and influence of anchoring peptide on function.  |  Hussain, MM. 1985. Biochim Biophys Acta. 815: 306-12. PMID: 2859892
  8. L-alanine-4-nitroanilide as a substrate for microsomal aminopeptidase.  |  Redondo, FL., et al. 1986. Clin Chem. 32: 912-3. PMID: 2870828
  9. Recommendation for the measurement of 'alanine aminopeptidase' in urine.  |  Mattenheimer, H., et al. 1988. J Clin Chem Clin Biochem. 26: 635-44. PMID: 2906650
  10. Substrate activation in the hydrolysis of L-alanine-4-nitroanilide catalyzed by aminopeptidase M.  |  Femfert, U. and Cichocki, P. 1972. FEBS Lett. 27: 219-20. PMID: 4664220
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  13. Dietary fiber viscosity and amino acid digestibility, proteolytic digestive enzyme activity and digestive organ weights in growing rats.  |  Larsen, FM., et al. 1994. J Nutr. 124: 833-41. PMID: 8207541

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

L-Alanine 4-nitroanilide, 1 g

sc-286050
1 g
$153.00

L-Alanine 4-nitroanilide, 5 g

sc-286050A
5 g
$612.00