kynureninase Antibody (E-5): sc-390360
- kynureninase Antibody (E-5) is a mouse monoclonal IgG1 κ kynureninase antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 104-308 mapping within an internal region of kynureninase of human origin
- kynureninase Antibody (E-5) is recommended for detection of kynureninase of human origin by WB, IP, IF and ELISA
- Anti-kynureninase Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for kynureninase Antibody (E-5) for WB applications. These reagents are now offered in bundles with kynureninase Antibody (E-5) (see ordering information below).
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kynureninase Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects kynureninase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). kynureninase (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Kynureninase, also known as L-kynurenine hydrolase, is a 465 amino acid enzyme predominantly located in the cytoplasm, where kynureninase plays a crucial role in tryptophan metabolism. This enzyme is essential for L-kynurenine degradation and NAD(+) cofactor biosynthesis, catalyzing L-kynurenine cleavage into anthranilic acid and L-3-hydroxykynurenine into 3-hydroxyanthranilic acid, with a notable preference for the L-3-hydroxy form. Kynureninase forms a homodimer structure that enhances stability and functionality, allowing for efficient substrate processing. Additionally, kynureninase utilizes pyridoxal phosphate as a cofactor and is inhibited by o-methoxybenzoylalanine (OMBA). Kynureninase is widely expressed in various tissues, with highest levels found in lung, placenta, and liver. A kynureninase deficiency can lead to hyperkynureninuria, a metabolic disorder characterized by impaired conversion of tryptophan to nicotinic acid (vitamin B6), while increased kynureninase activity has been associated with systemic and cerebral inflammatory conditions, highlighting kynureninase′s potential role in disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
kynureninase Antibody (E-5) References:
- Purification and biochemical characterization of some of the properties of recombinant human kynureninase. | Walsh, HA. and Botting, NP. 2002. Eur J Biochem. 269: 2069-74. PMID: 11985583
- HYDROXYKYNURENINURIA: A CASE OF ABNORMAL TRYPTOPHAN METABOLISM PROBABLY DUE TO A DEFICIENCY OF KYNURENINASE. | KOMROWER, GM., et al. 1964. Arch Dis Child. 39: 250-6. PMID: 14169454
- Xanthurenic aciduria due to a mutation in KYNU encoding kynureninase. | Christensen, M., et al. 2007. J Inherit Metab Dis. 30: 248-55. PMID: 17334708
- A mechanism of quinolinic acid formation by brain in inflammatory neurological disease. Attenuation of synthesis from L-tryptophan by 6-chlorotryptophan and 4-chloro-3-hydroxyanthranilate. | Heyes, MP., et al. 1993. Brain. 116 (Pt 6): 1425-50. PMID: 8293279
- Isolation and expression of a cDNA clone encoding human kynureninase. | Alberati-Giani, D., et al. 1996. Eur J Biochem. 239: 460-8. PMID: 8706755
- Congenital non-progressive encephalopathy and deafness with intermittent episodes of coma and hyperkynureninuria. | Cheminal, R., et al. 1996. J Inherit Metab Dis. 19: 25-30. PMID: 8830173
- Cloning and recombinant expression of rat and human kynureninase. | Toma, S., et al. 1997. FEBS Lett. 408: 5-10. PMID: 9180257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
kynureninase Antibody (E-5) | sc-390360 | 200 µg/ml | $322.00 | |||
kynureninase Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-530138 | 200 µg Ab; 10 µg BP | $361.00 | |||
kynureninase Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-523509 | 200 µg Ab, 40 µg BP | $361.00 | |||
kynureninase Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-543885 | 200 µg Ab; 20 µg BP | $361.00 | |||
kynureninase Antibody (E-5) AC | sc-390360 AC | 500 µg/ml, 25% agarose | $424.00 | |||
kynureninase Antibody (E-5) HRP | sc-390360 HRP | 200 µg/ml | $322.00 | |||
kynureninase Antibody (E-5) FITC | sc-390360 FITC | 200 µg/ml | $336.00 | |||
kynureninase Antibody (E-5) PE | sc-390360 PE | 200 µg/ml | $349.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 488 | sc-390360 AF488 | 200 µg/ml | $364.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 546 | sc-390360 AF546 | 200 µg/ml | $364.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 594 | sc-390360 AF594 | 200 µg/ml | $364.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 647 | sc-390360 AF647 | 200 µg/ml | $364.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 680 | sc-390360 AF680 | 200 µg/ml | $364.00 | |||
kynureninase Antibody (E-5) Alexa Fluor® 790 | sc-390360 AF790 | 200 µg/ml | $364.00 |