Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Kremen-1 CRISPR/Cas9 KO Plasmid (h): sc-403791
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Kremen-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Kremen-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Kremen-1 Antibody (R17-2): sc-74206
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Kremen-1 CRISPR/Cas9 KO Plasmid (h) | sc-403791 | 20 µg | $397.00 |
Overview
KREMEN1 encodes Kremen-1, a single-pass transmembrane receptor best known as a high-affinity binding partner for Dickkopf (DKK) proteins that modulates canonical Wnt/β-catenin signaling. By forming complexes with LRP5/6 and DKK1, Kremen-1 promotes receptor internalization and attenuates Wnt-driven transcriptional programs that control cell fate decisions, proliferation, and tissue homeostasis. Through this pathway linkage, KREMEN1 has been studied in contexts where Wnt pathway imbalance contributes to developmental phenotypes and disease-associated signaling rewiring, including cancer-related and bone-related research settings. Its membrane localization and receptor–ligand interactions also make it useful for dissecting pathway cross-talk at the cell surface and downstream β-catenin–dependent gene regulation.
Kremen-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KREMEN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KREMEN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KREMEN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Kremen-1 protein expression.
This CRISPR knockout system enables efficient generation of KREMEN1-deficient cell models for investigation of Kremen-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KREMEN1 exon(s) critical for Kremen-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KREMEN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Kremen-1 CRISPR/Cas9 KO Plasmid (h) and Kremen-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KREMEN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Kremen-1 HDR Plasmid (h) and Kremen-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KREMEN1 homology arms to support homology-directed repair at defined KREMEN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.