KNRK + PMA nuclear extract: sc-2142
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- rat nuclear extract; K-Ras transformed kidney cells induced with phorbol
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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KNRK + PMA nuclear extract combines components from the Kirsten rat sarcoma virus-transformed rat kidney epithelial cell line (KNRK) with Phorbol 12-myristate 13-acetate (PMA), a known activator of protein kinase C (PKC). This nuclear extract is pivotal in studying PKC-mediated signaling pathways and their effects on transcriptional regulation and cellular transformation in a research context. The addition of PMA to KNRK extract allows for a focused investigation into the role of PKC in modifying transcription factor activity, influencing chromatin structure, and regulating gene expression, particularly in pathways related to cell growth and oncogenesis. Researchers utilize this extract in a variety of molecular biology assays, such as chromatin immunoprecipitation (ChIP) to explore how activated PKC interacts with chromatin components, and electrophoretic mobility shift assays (EMSA) to assess changes in DNA-binding activities under conditions of PKC stimulation. This type of extract is essential for elucidating the complex mechanisms by which PKC alters nuclear signaling in transformed cells, providing insights into the basic biological processes. The integrity and origin of the KNRK cell line are carefully validated to ensure consistent and reliable results in these research endeavors.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KNRK + PMA nuclear extract | sc-2142 | 250 µg/0.05 ml | $160.00 |