KLHL25 Antibody (K-20): sc-100774

KLHL25 Antibody (K-20) is a mouse monoclonal IgG2a kappa light chain antibody that detects KLHL25 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-KLHL25 antibody (K-20) is available as a non-conjugated format. KLHL25, also known as Ectoderm-neural cortex protein 2 (ENC2), is a cytoplasmic protein characterized by six Kelch repeats and a single BTB (POZ) domain, which are crucial for cellular processes. KLHL25 is highly homologous to another Kelch-like protein, ENC1, and operates similarly to other Kelch-domain containing proteins, which often serve as modifiers of actin fibers. KLHL25 expression is particularly prominent during early embryogenesis, playing a significant role in neuronal process formation and neural crest cell differentiation. KLHL25 functions as a substrate-specific adapter for protein ubiquitinating complexes, highlighting its importance in regulating protein degradation pathways. KLHL25 is expressed in various tissues, with highest levels found in brain and liver, underscoring its potential involvement in critical physiological processes within these organs.

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KLHL25 Antibody (K-20) References:

1. The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation.  |  Angers, S., et al. 2006. Nat Cell Biol. 8: 348-57. PMID: 16547521
2. Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast.  |  Peeters, T., et al. 2006. Proc Natl Acad Sci U S A. 103: 13034-9. PMID: 16924114
3. KLHL1/MRP2 mediates neurite outgrowth in a glycogen synthase kinase 3beta-dependent manner.  |  Seng, S., et al. 2006. Mol Cell Biol. 26: 8371-84. PMID: 16982692
4. The Kelch-like protein 1 modulates P/Q-type calcium current density.  |  Aromolaran, KA., et al. 2007. Neuroscience. 145: 841-50. PMID: 17289272
5. The BTB-Kelch protein KLEIP controls endothelial migration and sprouting angiogenesis.  |  Nacak, TG., et al. 2007. Circ Res. 100: 1155-63. PMID: 17395875
6. Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange.  |  Niranjan, T., et al. 2007. J Biol Chem. 282: 24231-8. PMID: 17584745
7. Directly from Galpha to protein kinase A: the kelch repeat protein bypass of adenylate cyclase.  |  Peeters, T., et al. 2007. Trends Biochem Sci. 32: 547-54. PMID: 17983752
8. Ectromelia virus BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3-based ubiquitin ligases.  |  Wilton, BA., et al. 2008. Virology. 374: 82-99. PMID: 18221766
9. BTB Protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase.  |  Rondou, P., et al. 2008. J Biol Chem. 283: 11083-96. PMID: 18303015
10. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein.  |  Hernandez, MC., et al. 1997. J Neurosci. 17: 3038-51. PMID: 9096139

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-KLHL25 Antibody (K-20) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-KLHL25 Antibody (K-20) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

KLHL25 Antibody (K-20) Specifications

Alternate Product Name: KLHL25

Product Clone Name: K-20

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-590

Epitope Target: KLHL25

Epitope Species: human

Product Reactivity/Detection: KLHL25

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for KLHL25 Antibody (K-20): N/A