KLHL1 Antibody (3A8B3): sc-517225
- KLHL1 Antibody (3A8B3) is a mouse monoclonal IgG1 KLHL1 antibody provided at 100 µg/ml
- raised against a recombinant protein corresponding to amino acids 202-506 of KLHL1 of human origin
- recommended for detection of KLHL1 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for KLHL1 Antibody (3A8B3). This work is in progress.
KLHL1 Antibody (3A8B3) is a mouse monoclonal IgG1 antibody that detects KLHL1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KLHL1 (3A8B3) antibody is available as a non-conjugated format. KLHL1 protein, also known as kelch-like protein 1, consists of 748 amino acids and serves as a homolog of the Drosophila kelch protein. KLHL1 is primarily localized to the cytoskeleton and features six kelch repeats along with one BTB (Broad-Complex, Tramtrack and Bric a brac) domain, which is crucial for actin organization. The BTB domain facilitates homodimerization and is essential for structural integrity, allowing interaction with various partners involved in cytoskeletal dynamics. KLHL1 is highly expressed in the brain, where KLHL1 plays a significant role in modulating neurite outgrowth, thereby influencing neuronal development and function. Expression of KLHL1 can be downregulated by the spinocerebellar ataxia type 8 (SCA8) protein, which is implicated in the neuropathogenesis of SCA8, a disorder characterized by symptoms such as limb and truncal ataxia, ataxic dysarthria, and horizontal nystagmus. KLHL1′s ability to interact with other proteins involved in cytoskeletal organization underscores KLHL1′s importance in maintaining neuronal health and function, making anti-KLHL1 antibody (3A8B3) a valuable tool for researchers studying neurodegenerative diseases and cytoskeletal dynamics.
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KLHL1 Antibody (3A8B3) References:
- The kelch repeat superfamily of proteins: propellers of cell function. | Adams, J., et al. 2000. Trends Cell Biol. 10: 17-24. PMID: 10603472
- The SCA8 transcript is an antisense RNA to a brain-specific transcript encoding a novel actin-binding protein (KLHL1). | Nemes, JP., et al. 2000. Hum Mol Genet. 9: 1543-51. PMID: 10888605
- In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions. | Melnick, A., et al. 2000. Mol Cell Biol. 20: 6550-67. PMID: 10938130
- Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals. | Prag, S. and Adams, JC. 2003. BMC Bioinformatics. 4: 42. PMID: 13678422
- KLHL1/MRP2 mediates neurite outgrowth in a glycogen synthase kinase 3beta-dependent manner. | Seng, S., et al. 2006. Mol Cell Biol. 26: 8371-84. PMID: 16982692
- Targeted deletion of a single Sca8 ataxia locus allele in mice causes abnormal gait, progressive loss of motor coordination, and Purkinje cell dendritic deficits. | He, Y., et al. 2006. J Neurosci. 26: 9975-82. PMID: 17005861
- The Kelch-like protein 1 modulates P/Q-type calcium current density. | Aromolaran, KA., et al. 2007. Neuroscience. 145: 841-50. PMID: 17289272
- Process elongation of oligodendrocytes is promoted by the Kelch-related protein MRP2/KLHL1. | Jiang, S., et al. 2007. J Biol Chem. 282: 12319-29. PMID: 17324934
- SCA8 mRNA expression suggests an antisense regulation of KLHL1 and correlates to SCA8 pathology. | Chen, WL., et al. 2008. Brain Res. 1233: 176-84. PMID: 18708037
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLHL1 Antibody (3A8B3) | sc-517225 | 100 µg/ml | $322.00 |