KIAA1429 Double Nickase Plasmid (h): sc-410574-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA1429 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KIAA1429 Double Nickase Plasmid (h) and KIAA1429 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KIAA1429. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA1429 Double Nickase Plasmid (h) | sc-410574-NIC | 20 µg | $410.00 |
KIAA1429 (also known as VIRMA) is a core component of the mRNA N6-methyladenosine (m6A) writer machinery and helps define methylation topology by promoting deposition near the 3′ UTR and stop codon regions. Through interactions with METTL3/METTL14/WTAP and related cofactors, KIAA1429 influences pre-mRNA processing, alternative polyadenylation, mRNA stability, and translation, thereby shaping transcriptome-wide gene expression programs. These RNA regulatory functions connect KIAA1429 to pathways governing cell-cycle progression, differentiation, and stress-adaptive responses. Dysregulated KIAA1429 expression or activity has been reported across multiple disease-relevant contexts, including oncogenic phenotypes, making it a frequently studied node in epitranscriptomic regulation.
KIAA1429 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KIAA1429 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KIAA1429. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KIAA1429 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KIAA1429-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.