Date published: 2026-10-22

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Ki67 Antibody (MIB-1): sc-101861

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Datasheets
  • Ki67 Antibody (MIB-1) is a mouse monoclonal IgG1, cited in 62 publications, provided at 1 ml supernatant
  • raised against nuclear fractions of Hodgkin's lymphoma cell line, L248 of human origin
  • recommended for detection of Ki67 of human origin by IF and IHC(P)
  • See Ki67 (Ki-67): sc-23900 for Ki67 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ki67 Antibody (MIB-1). This work is in progress.
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Ki67 Antibody (MIB-1) is a mouse monoclonal IgG1 antibody that detects Ki67 of human origin by immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Ki67 antibody (MIB-1) is available as the non-conjugated form. Ki67 is a crucial nuclear protein expressed exclusively in actively dividing cells, making Ki67 an essential marker for assessing cell proliferation in various solid tumors and hematological malignancies. Ki67 expression levels correlate with the histopathological grade of neoplasms, providing valuable insights into tumor aggressiveness and potential treatment outcomes. Assessment of Ki67 expression in renal and ureter tumors has demonstrated a direct correlation between tumor proliferation and disease progression, enabling clinicians to identify high-risk patients more effectively. Ki67 expression is instrumental in differentiating between malignant and benign peripheral nerve sheath tumors, underscoring Ki67′s importance in cancer diagnostics and prognostics.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ki67 Antibody (MIB-1) References:

  1. Ki67 for evaluating the prognosis of gastrointestinal stromal tumors: A systematic review and meta-analysis.  |  Li, J., et al. 2022. Oncol Lett. 23: 189. PMID: 35527778
  2. The value of Ki67 for the diagnosis of LSIL and the problems of p16 in the diagnosis of HSIL.  |  Liu, J., et al. 2022. Sci Rep. 12: 7613. PMID: 35534530
  3. Association between Ki67 expression and therapeutic outcome in colon cancer.  |  Liu, Q., et al. 2023. Oncol Lett. 25: 272. PMID: 37216165
  4. A nomogram incorporating Ki67 to predict survival of acral melanoma.  |  Du, Y., et al. 2023. J Cancer Res Clin Oncol. 149: 13077-13085. PMID: 37470854

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ki67 Antibody (MIB-1)

sc-101861
1 ml supernatant
$322.00

What is clone number for this Mab?

Asked by: nini
感谢您的提问。 该产品克隆号是MIB-1。 Thank you for your question. The clone number is MIB-1.
Answered by: Ling Chen
Date published: 2024-10-17

what is the recommended dilution for IHC please?

Asked by: Esteban
Thank you for your question. 1:50-1:500 https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: BlakeJ
Date published: 2023-12-18

What is the concentration of this antibody?

Asked by: MDW47
Thank you for your question. This antibody is provided as cultured supernatant and the exact concentration of IgG has not been determined. Please see our product data sheet for our recommended usage instructions under the applications.
Answered by: Technical Support
Date published: 2019-01-31

Is this Antibody cross react with mouse antigen? I am going to do Paraffin embedded tissue IHC.

Asked by: Jeongin
Thank you for your question. We do not currently recommend this antibody, Ki-67 (MIB-1): sc-101861, for detection of mouse Ki-67 because we have not yet tested the antibody for cross-reactivity with this species.
Answered by: Technical Support
Date published: 2017-06-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-08-30

Will this antibody react with bovine tissue

Asked by: immuno
Thank you for your question. It is unknown whether or not this antibody can react with bovine samples. Ki-67 Antibody (MIB-1): sc-101861 is raised against nuclear fractions of hodgkin's lymphoma cell line, L248 of human origin. The exact epitope recognized by this clone is unknown. Therefore, we can not predict cross-reactivity with bovine samples based on sequence homology. And we have not tested the antibody in bovine, nor are we aware of any publications in bovine. Unfortunately, we do not offer any Ki-67 antibodies at this time which are recommended for use in bovine.
Answered by: Technical Support
Date published: 2017-01-06
  • y_2026, m_10, d_21, h_6CST
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Rated 5 out of 5 by Batool from Great antibody for IF proliferationWe regularly use this antibody in IF as a proliferation marker. The results are reliable and specific.
Date published: 2024-08-07
Rated 5 out of 5 by DefinitelyNotMatt from Reliable Antibody, Great for IHCThis antibody is great for IF and tissue staining, high specificity and low background signal.
Date published: 2017-01-13
Rated 1 out of 5 by Kama from Doesn't work for IHC at allTried this antibody for immunohistochemistry FFPE tissues with known controls and didn't turn up.
Date published: 2016-12-04
Rated 5 out of 5 by Minhui_Hua from The antibody worked well in WB or IHCThe antibody worked well in WB or IHC.
Date published: 2015-11-05
Rated 5 out of 5 by SF from Published IHC data using SCPublished IHC data using SC-101861 with human soft tissue sections. -SCBT Publication Review
Date published: 2013-06-24
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Ki67 Antibody (MIB-1) is rated 4.2 out of 5 by 5.
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