Keratin 32 CRISPR/Cas9 KO Plasmid (h): sc-410857
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Keratin 32 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Keratin 32 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Keratin 32 CRISPR/Cas9 KO Plasmid (h) | sc-410857 | 20 µg | $397.00 |
Overview
KRT32 encodes keratin 32, a type I hair keratin that polymerizes with type II keratins to form intermediate filaments essential for the structural integrity and mechanical resilience of hair fibers. As part of the keratin cytoskeleton, KRT32 contributes to cytoskeletal organization, epithelial differentiation programs in hair follicle lineages, and the assembly of keratinized structures. Altered expression or disruption of hair keratin networks is associated with hair shaft fragility phenotypes and provides a tractable readout for studying keratin filament assembly dynamics. KRT32 is therefore relevant for investigating cytoskeletal stress responses, keratin filament turnover, and genotype–phenotype relationships in hair biology and keratinization processes.
Keratin 32 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KRT32 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KRT32 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KRT32 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Keratin 32 protein expression.
This CRISPR knockout system enables efficient generation of KRT32-deficient cell models for investigation of Keratin 32 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KRT32 exon(s) critical for Keratin 32 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KRT32 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Keratin 32 CRISPR/Cas9 KO Plasmid (h) and Keratin 32 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KRT32 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Keratin 32 HDR Plasmid (h) and Keratin 32 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KRT32 homology arms to support homology-directed repair at defined KRT32 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.