Molecular structure of Kemptide, CAS Number: 65189-71-1
Kemptide (CAS 65189-71-1)
Application:
Kemptide is a phosphate acceptor peptide
CAS Number:
65189-71-1
Purity:
≥97%
Molecular Weight:
771.92
Molecular Formula:
C32H61N13O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Kemptide, phosphate acceptor, is shown to be a basic serine-containing heptapeptide corresponding to a sequence from pig liver pyruvate kinase. This synthetic peptide is a substrate of cyclic AMP-dependent protein kinase and is phosphorylated and activated after microinjection into Xenopus oocytes. Exposure of Kemptide to insulin has demonstrated an increase in activation of this agent, which can be inhibited by the catalytic subunit of phosphatase 2A.
Kemptide (CAS 65189-71-1) References
- Enzyme assay for protein kinase using micellar electrokinetic chromatography with laser-induced fluorescence detection. | Sano, M., et al. 2003. J Chromatogr B Analyt Technol Biomed Life Sci. 794: 149-56. PMID: 12888207
- An insulin-stimulated kemptide kinase purified from rat liver is deactivated by phosphatase 2A. | Klarlund, JK., et al. 1991. J Biol Chem. 266: 4052-5. PMID: 1847913
- TiO2-assisted silver enhanced biosensor for kinase activity profiling. | Ji, J., et al. 2009. Chem Commun (Camb). 1508-10. PMID: 19277372
- Studies on the mechanism of phosphorylation of synthetic polypeptides by a calf thymus cyclic AMP-dependent protein kinase. | Pomerantz, AH., et al. 1977. Proc Natl Acad Sci U S A. 74: 4261-5. PMID: 200911
- In vivo phosphorylation of a synthetic peptide substrate of cyclic AMP-dependent protein kinase. | Maller, JL., et al. 1978. Proc Natl Acad Sci U S A. 75: 248-51. PMID: 203933
- Determination of soluble cAMP-dependent protein kinase activity in guinea-pig tracheal smooth muscle. Preferential use of kemptide as a phosphorylating substrate. | Langlands, JM. and Rodger, IW. 1990. Biochem Pharmacol. 39: 1365-74. PMID: 2322318
- Molecular dynamics simulation studies on the positive cooperativity of the Kemptide substrate with protein kinase A induced by the ATP ligand. | Li, C., et al. 2014. J Phys Chem B. 118: 1273-87. PMID: 24456306
- Study of the affinity between the protein kinase PKA and peptide substrates derived from kemptide using molecular dynamics simulations and MM/GBSA. | Mena-Ulecia, K., et al. 2014. PLoS One. 9: e109639. PMID: 25275314
- Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. | Kübler, D., et al. 1989. J Biol Chem. 264: 14549-55. PMID: 2547801
- A QM/MM study of Kemptide phosphorylation catalyzed by protein kinase A. The role of Asp166 as a general acid/base catalyst. | Pérez-Gallegos, A., et al. 2015. Phys Chem Chem Phys. 17: 3497-511. PMID: 25535906
- Substrate phosphorylation can inhibit proteolysis by trypsin-like enzymes. | Benore-Parsons, M., et al. 1989. Arch Biochem Biophys. 272: 274-80. PMID: 2751304
- Highly sensitive photoelectrochemical biosensor for kinase activity detection and inhibition based on the surface defect recognition and multiple signal amplification of metal-organic frameworks. | Wang, Z., et al. 2017. Biosens Bioelectron. 97: 107-114. PMID: 28582705
- Study of the affinity between the protein kinase PKA and homoarginine-containing peptides derived from kemptide: Free energy perturbation (FEP) calculations. | Mena-Ulecia, K., et al. 2018. J Comput Chem. 39: 986-992. PMID: 29399821
- Vanadium oxoanions and cAMP-dependent protein kinase: an anti-substrate inhibitor. | Pluskey, S., et al. 1997. Biochem J. 321 (Pt 2): 333-9. PMID: 9020863
- TECHNICAL NOTE: measurement of cAMP-dependent protein kinase activity using a fluorescent-labeled kemptide. | MacAla, LJ., et al. 1998. Kidney Int. 54: 1746-50. PMID: 9844154
Substrate of:
Enzyme, and PKA.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Kemptide, 1 mg | sc-201153 | 1 mg | $45.00 |